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Rat Adiponectin/Acrp30 ELISA Kit (Colorimetric) Summary
Description
Adipoq (Rat) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of rat adipoq in urine, plasma, serum, and cell culture supernatants.
Biotinylated Human alpha-Fetoprotein Antibody (50x): A 50-fold concentratedbiotinylated polyclonal antibody against human alpha-fetoprotein.
Chromogen Substrate (1x): A stabilized peroxidase chromogen substratetetramethylbenzidine.
Human alpha-Fetoprotein Microplate: A 96-well polystyrene microplate (12 strips of 8wells) coated with a polyclonal antibody against human alpha-fetoprotein.
Human alpha-Fetoprotein Standard: Human alpha-fetoprotein in a buffered proteinbase, lyophilized.
MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
Sealing Tapes: Pressure sensitive sealing tapes that can be cut to fit the format of theindividual assay.
SP Conjugate (100x): A 100-fold concentrate.
Stop Solution (1x): A 0.5 N hydrochloric acid solution to stop the chromogen substratereaction.
Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Rat Adiponectin/Acrp30 ELISA Kit (Colorimetric)
ACDC
Acrp30
ACRP30ADPN
adipocyte, C1Q and collagen domain containing
Adiponectin
adiponectin, C1Q and collagen domain containing
AdipoQ
ADIPQTL1
ApM1
apM-1
APM1APM-1
C1q and collagen domain-containing protein
GBP28
GBP28apM1
Gelatin-binding protein
Background
Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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