Human Adiponectin/Acrp30 ELISA Kit (Colorimetric) Summary
Description |
Quality control test: Standard curve. ADIPOQ (Human) High Sensitivity ELISA Kit is a sandwich enzymelinked immunosorbent assay for the quantitative measurement of human adiponectin. |
Standard Curve Range |
1 to 100 ng/mL |
Sensitivity |
0.47 ng/mL (serum, plasma), 0.156 ng/mL (urine, CSF) |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 6.00% |
Intra-Assay |
CV% < 3.90% |
Spike Recovery |
90.50% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ADIPOQ |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
Components
|
- Antibody Coated Microtiter Strips
- Conjugate Solution
- Dilution Buffer Conc. (10x)
- Quality Control HIGH
- Quality Control LOW
- Set of Standards
- Stop Solution
- Substrate Solution
- Wash Solution Conc. (10x)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Adiponectin/Acrp30 ELISA Kit (Colorimetric)
Background
Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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