Adenylosuccinate Lyase Antibody (16C10) - BSA Free

Western Blot: Adenylosuccinate Lyase Antibody (16C10) [NBP2-59414] - Lane 1: Recombinant Protein, Lane 2: HeLa cell lysates, Lane 3: 293T cell lysates Lane 4: Jurkat cell lysates, Lane 5: HepG2 cell lysates Lane 6: A549 cell lysates Lane 7: MCF7 cell lysates

Immunocytochemistry/Immunofluorescence: Adenylosuccinate Lyase Antibody (16C10) [NBP2-59414] - Analysis of ADSL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ADSL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: Adenylosuccinate Lyase Antibody (16C10) [NBP2-59414] - Analysis of ADSL in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 16C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Adenylosuccinate Lyase Antibody (16C10) - BSA Free Summary

• Description: Novus Biologicals Mouse Adenylosuccinate Lyase Antibody (16C10) - BSA Free (NBP2-59414) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Adenylosuccinate Lyase (1-484aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADSL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1024
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Adenylosuccinate Lyase Antibody (16C10) - BSA Free

• adenylosuccinase
• adenylosuccinate lyase
• AMPS
• ASase
• ASL
• EC 4.3.2
• EC 4.3.2.2

Background

Adenylsuccinate lyase is involved in both de novo synthesis of purines and formation of adenosine monophosphate from inosine monophosphate. It catalyzes two reactions in AMP biosynthesis: the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and removal of fumarate from adenylosuccinate to give AMP. Adenylosuccinase deficiency results in succinylpurinemic autism, psychomotor retardation, and , in some cases, growth retardation associated with muscle wasting and epilepsy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADSL