GART Antibody (4D6-1D5)

Images

 
Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of GART expression in transfected 293T cell line by GART monoclonal antibody (M01), clone 4D6-1D5.Lane 1: GART transfected lysate(46 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of monoclonal antibody to GART on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of monoclonal antibody to GART on formalin-fixed paraffin-embedded human endometrium tissue. Antibody concentration 2 ug/ml.
Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - GART monoclonal antibody (M01), clone 4D6-1D5 Analysis of GART expression in Hela S3 NE.
Immunoprecipitation: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of GART transfected lysate using anti-GART monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GART MaxPab rabbit polyclonal ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, PLA
Clone
4D6-1D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GART Antibody (4D6-1D5) Summary

Immunogen
GART (AAH38958.1, 1 a.a. ~ 433 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLTKNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLDGLLCTSLPVWLENHTALTVVMASKGYPGDYTKGVEITGFPEAQALGLEVFHAGTALKNGKVVTHGGRVLAVTAIRENLISALEEAKKGLAAIKFEGAIYRKDVGFRAIAFLQQPR
Specificity
GART - phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GART
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against recominant protein, transfected lysate and cell lysate for WB. It has been used for IF, IHC-P and ELISA. Use in PLA reported in secitific publication PMID: 32439803
Publications
Read Publications using
H00002618-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GART Antibody (4D6-1D5)

  • AIRS
  • EC 6.3.3.1
  • EC 6.3.4.13
  • GARS
  • GARTF
  • MGC47764
  • PGFTEC 2.1.2.210PAIS
  • phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamidesynthetase, phosphoribosylaminoimidazole synthetase
  • trifunctional purine biosynthetic protein adenosine-3

Background

The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GART Antibody (H00002618-M01)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, PLA.


Filter By Application
ICC/IF
(2)
PLA
(1)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 9 of 9.
Publications using H00002618-M01 Applications Species
Doigneaux C, Pedley AM, Mistry IN et al. Hypoxia Drives the Assembly of the Multi-Enzyme Purinosome Complex J. Biol. Chem. May 21 2020 [PMID: 32439803] (PLA, Human) PLA Human
Severi L, Losi L, Fonda S et al. Proteomic and Bioinformatic Studies for the Characterization of Response to Pemetrexed in Platinum Drug Resistant Ovarian Cancer. Front Pharmacol May 8 2018 [PMID: 29867465]
Pedley AM, Benkovic SJ. Detecting Purinosome Metabolon Formation with Fluorescence Microscopy. Methods Mol. Biol. Apr 2 2018 [PMID: 29605921] (ICC/IF, Human) ICC/IF Human
Baresova V, Krijt M, Skopova V et al. CRISPR-Cas9 induced mutations along de novo purine synthesis in HeLa cells result in accumulation of individual enzyme substrates and affect purinosome formation. Mol Genet Metab 2016 Aug 24 [PMID: 27590927]
Genovese F, Gualandi A, Taddia L et al. Mass Spectrometric/Bioinformatic Identification of a Protein Subset That Characterizes the Cellular Activity of Anticancer Peptides. J Proteome Res. 2014 Sep 29 [PMID: 25196676]
Zhao A, Tsechansky M, Swaminathan J et al. Transiently transfected purine biosynthetic enzymes form stress bodies. PLoS One. 2013 Feb 6 [PMID: 23405267] (ICC/IF, Human) ICC/IF Human
Banerjee D, Ghosh D, Chatterjee A et al. No Evidence for Mutations that Deregulate GARS-AIRS-GART Protein Levels in Children with Down Syndrome. Ind J Clin Biochem. 2012 Jan [PMID: 23277712]
Noelker C, Schwake M, Balzer-Geldsetzer M et al. Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease. Neurosci Lett. 2011 Dec 01 [PMID: 22155091]
Field MS, Anguera MC, Page R, Stover PJ. 5,10-Methenyltetrahydrofolate Synthetase Activity is Increased in Tumors and Modifies the Efficacy of Antipurine LY309887. Arch Biochem Biophys. 2008 Nov 17 [PMID: 19022216]

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Product General Protocols

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Bioinformatics

Gene Symbol GART
Entrez
OMIM
Uniprot