Acyloxyacyl Hydrolase Antibody - BSA Free

Western Blot: Acyloxyacyl Hydrolase Antibody [NBP2-33905] - Analysis in control (vector only transfected HEK293T lysate) and AOAH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry: Acyloxyacyl Hydrolase Antibody [NBP2-33905] - Staining of human nasopharynx shows strong cytoplasmic positivity in respiratory epithelial cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Acyloxyacyl Hydrolase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Acyloxyacyl Hydrolase Antibody - BSA Free (NBP2-33905) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: VKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSVPFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKDGVPYEKKFCEGSQPRGIILLGDSAGAH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AOAH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Acyloxyacyl Hydrolase Protein (NBP2-33905PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Acyloxyacyl Hydrolase Antibody - BSA Free

• acyloxyacyl hydrolase (neutrophil)
• acyloxyacyl hydrolase
• EC 3.1.1.77

Background

Acyloxyacyl Hydrolase, also known as AOAH, contains a 65 kDa and a 62 kDa isoform, and is involved in the detoxification of bacterial lipopolysaccharides by eliminating fatty acyl chains from the molecule. Current research is being conducted on Acyloxyacl Hydrolase and its relation to the common cold, hepatitis, gastric cancer, osteochondrosis, asthma, and mastitis. The protein is linked to inflammatory responses and lipid metabolic process where it interacts with IGHG1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AOAH
• Uniprot:
  • P28039(Human)