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Dopa Decarboxylase/DDC Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse kidney tissue, PC-12 rat adrenal pheochromocytoma cell line, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC ...read more
Dopa Decarboxylase/DDC was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 10 ...read more
Dopa Decarboxylase/DDC was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody ...read more
Dopa Decarboxylase/DDC was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 5 µg/mL ...read more
DOPA decarboxylase is expressed non-uniformly in the CNS vasculature.(A–F) Coronal section of a P7 C57BL/6 brain stained for either GS lectin (green) to mark blood vessels or DOPA decarboxylase (DDC; magenta). ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Dopa Decarboxylase/DDC Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dopa Decarboxylase/DDC
Met1-Glu480
Accession # P20711
Specificity
Detects human Dopa Decarboxylase/DDC in direct ELISAs and human, mouse and rat Dopa Decarboxylase/DDC in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDC
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3564 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dopa Decarboxylase/DDC Antibody [Unconjugated]

  • AADC
  • AADCaromatic-L-amino-acid decarboxylase
  • DDC
  • dopa decarboxylase (aromatic L-amino acid decarboxylase)
  • Dopa Decarboxylase
  • EC 4.1.1
  • EC 4.1.1.28

Background

Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).

  1. Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
  2. Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dopa Decarboxylase/DDC Antibody (AF3564)(4)

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Bioinformatics

Gene Symbol DDC
Uniprot