Dopa Decarboxylase/DDC Antibody [Unconjugated]

Western blot shows lysates of mouse kidney tissue, PC-12 rat adrenal pheochromocytoma cell line, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Dopa Decarboxylase/DDC was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Dopa Decarboxylase/DDC was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Dopa Decarboxylase/DDC was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

DOPA decarboxylase is expressed non-uniformly in the CNS vasculature.(A–F) Coronal section of a P7 C57BL/6 brain stained for either GS lectin (green) to mark blood vessels or DOPA decarboxylase (DDC; magenta). Rightmost column shows merged images. Images are from the same brain section and correspond to cortex (A and B), striatum (C and D), and basal forebrain (E and F). DDC immunoreactivity is observed in blood vessels and in axon terminals, and in the latter it appears as distinct puncta in the cortex or more concentrated staining in the striatum. Yellow arrows in (C) indicate a large vein. Str, striatum; Ctx, cortex; AC, anterior commissure; Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30188322), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Dopa Decarboxylase/DDC Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dopa Decarboxylase/DDC
  Met1-Glu480
  Accession # P20711
• Specificity: Detects human Dopa Decarboxylase/DDC in direct ELISAs and human, mouse and rat Dopa Decarboxylase/DDC in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DDC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dopa Decarboxylase/DDC Antibody [Unconjugated]

• AADC
• AADCaromatic-L-amino-acid decarboxylase
• DDC
• dopa decarboxylase (aromatic L-amino acid decarboxylase)
• Dopa Decarboxylase
• EC 4.1.1
• EC 4.1.1.28

Background

Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).

1. Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
2. Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dopa Decarboxylase/DDC Antibody (AF3564)(4)

Publications using AF3564

• SV Beliakov, V Blokhin, SA Surkov, MV Ugrumov LUHMES Cells: Phenotype Refinement and Development of an MPP+-Based Test System for Screening Antiparkinsonian Drugs International Journal of Molecular Sciences, 2023-01-01;24(1):. 2023-01-01 [PMID: 36614176] (ICC/IF, Human)
• D Troshev, A Bannikova, V Blokhin, A Kolacheva, T Pronina, M Ugrumov Striatal Neurons Partially Expressing a Dopaminergic Phenotype: Functional Significance and Regulation International Journal of Molecular Sciences, 2022-09-21;23(19):. 2022-09-21 [PMID: 36232359] (IHC, Mouse)
• El-Shehawy R, Luecke-Johansson S, Ribbenstedt A, Gorokhova E Microbiota-Dependent and -Independent Production of l-Dopa in the Gut of Daphnia magna mSystems 11/9/2021 [PMID: 34751589]
• Mark F Sabbagh, Jacob S Heng, Chongyuan Luo, Rosa G Castanon, Joseph R Nery, Amir Rattner et al. Transcriptional and epigenomic landscapes of CNS and non-CNS vascular endothelial cells eLife 2018-09-06 [PMID: 30188322]

Bioinformatics

• Gene Symbol: DDC
• Uniprot:
  • P20711()