ACTL6B Antibody - BSA Free

Western Blot: ACTL6B Antibody [NBP2-15269] - Sample (30 ug of whole cell lysate) A: SK-N-SH 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ACTL6B Antibody [NBP2-15269] - Paraformaldehyde-fixed HeLa, using Actin like 6B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

BAF53B antibody detects BAF53B protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BAF53B stained by BAF53B antibody (NBP2-15269) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Rt, Mu, Bv, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ACTL6B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ACTL6B Antibody - BSA Free (NBP2-15269) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-ACTL6B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ACTL6B. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (99%), Rhesus Macaque (100%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTL6B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ACTL6B Antibody - BSA Free

• 53 kDa BRG1-associated factor B
• actin-like 6
• actin-like 6B
• Actin-related protein Baf53b
• ACTL6
• arpNalpha
• BAF53Bactin-like protein 6B
• BRG1-associated factor 53B
• hArpN alpha

Background

The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ACTL6B Antibody (NBP2-15269)(1)

Publication using NBP2-15269

• Yongli Shan, Yanqi Zhang, Yuan Zhao, Tianyu Wang, Jingyuan Zhang, Jiao Yao, Ning Ma, Zechuan Liang, Wenhao Huang, Ke Huang, Tian Zhang, Zhenghui Su, Qianyu Chen, Yanling Zhu, Chuman Wu, Tiancheng Zhou, Wei Sun, Yanxing Wei, Cong Zhang, Chenxu Li, Shuquan Su, Baojian Liao, Mei Zhong, Xiaofen Zhong, Jinfu Nie, Duanqing Pei, Guangjin Pan JMJD3 and UTX determine fidelity and lineage specification of human neural progenitor cells Nature Communications 2020-01-20 [PMID: 31959746]

Bioinformatics

• Gene Symbol: ACTL6B
• Uniprot:
  • O94805(Human)