Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: BAF53A Antibody [NB100-61628] - Western blot of WT and ARID1A-/- HCT116 nuclear extracts separated by glycerol gradient centrifugation. BAF53A antibody (NB100-61628) used for blotting. Representative of ...read more
Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: BAF53A Antibody [NB100-61628] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-BAF53A ...read more
Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF reactivity reported in (PMID: 23395444). Use in IHC reported in scientific literature (PMID:33469301)
47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-61628 in the following applications:
actin-related protein 4,53 kDa BRG1-associated factor A
Actin-related protein Baf53a
BAF complex 53 kDa subunit
BRG1-associated factor 53A
INO80 complex subunit K
BAF53A is a 53kd subunit of the BAF (BRG1/brm-associated factor) complex, a SWI/SNF chromatin remodeling and histone acetyltransferase complex that functions in the regulation of gene transcription. In the BAF complex, BAF53A is important to the ATPase activity of SMARCA4/BRG1 and the association of the BAF complex with chromatin. BAF53A also appears to be important to the formation of higher-order chromatin structure and has been shown to associate with mitotic chromosomes. Recently, BAF53A has been found to interact with p53 and repress p53-mediated gene transcription in a BRG1-independent manner.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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