BAF53A Antibody

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Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: BAF53A Antibody [NB100-61628] - Western blot of WT and ARID1A-/- HCT116 nuclear extracts separated by glycerol gradient centrifugation. BAF53A antibody (NB100-61628) used for blotting. Representative of ...read more
Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: BAF53A Antibody [NB100-61628] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-BAF53A ...read more
Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB
BRD9 defines a distinct, non-canonical BAF complex. c) Immunoprecipitation (IP) experiments from mouse ESC nuclear lysates using antibodies against BRG1, BRD9, and BAF47. Blots developed using chemiluminescence are ...read more
Knockdown of ARID1B is synthetically lethal with ARID1A deletion in HCT116 cells. (D) Western blot of WT and ARID1A-/- HCT116 nuclear extracts separated by glycerol gradient centrifugation. Antibodies used for blotting ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

BAF53A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human BRG1-associated factor, 53 kDa subunit A using the numbering given in entry NP_004292.1 (GeneID 86).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTL6A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Simple Western 1:40
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
ICC/IF reactivity reported in (PMID: 23395444). Use in IHC reported in scientific literature (PMID:33469301)
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-61628 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BAF53A Antibody

  • actin-like 6A
  • actin-like protein 6A
  • actin-related protein 4,53 kDa BRG1-associated factor A
  • Actin-related protein Baf53a
  • Actl6
  • Arp4
  • arpNbeta
  • BAF complex 53 kDa subunit
  • Baf53a
  • BAF53AACTL6
  • BAF53MGC5382
  • BRG1-associated factor 53A
  • hArpN beta
  • INO80 complex subunit K
  • INO80KARPN-BETA

Background

BAF53A is a 53kd subunit of the BAF (BRG1/brm-associated factor) complex, a SWI/SNF chromatin remodeling and histone acetyltransferase complex that functions in the regulation of gene transcription. In the BAF complex, BAF53A is important to the ATPase activity of SMARCA4/BRG1 and the association of the BAF complex with chromatin. BAF53A also appears to be important to the formation of higher-order chromatin structure and has been shown to associate with mitotic chromosomes. Recently, BAF53A has been found to interact with p53 and repress p53-mediated gene transcription in a BRG1-independent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAF53A Antibody (NB100-61628)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IF/IHC, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(2)
WB
(6)
All Applications
Filter By Species
Human
(5)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-61628 Applications Species
Wischhof L, Lee HM, Tutas J et al. BCL7A-containing SWI/SNF/BAF complexes modulate mitochondrial bioenergetics during neural progenitor differentiation The EMBO Journal 2022-12-01 [PMID: 36305367]
Chang CY, Shipony Z, Lin SG Et al. Increased ACTL6A occupancy within mSWI/SNF chromatin remodelers drives human squamous cell carcinoma Molecular cell 2021-10-19 [PMID: 34687603]
Braun S, Petrova R, Tang J et al. The npBAF to nBAF Chromatin Switch Regulates Cell Cycle Exit in the Developing Mammalian Cortex Genes Dev 2021-02-19 [PMID: 33602870]
Gatchalian J, Malik S, Ho J et al. A non-canonical BRD9-containing BAF chromatin remodeling complex regulates naive pluripotency in mouse embryonic stem cells Nat Commun 2018-12-03 [PMID: 30510198] (WB, Mouse) WB Mouse
Kelso TWR, Porter DK, Amaral ML et al. Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers. Elife. 2017-10-02 [PMID: 28967863] (WB, Human) WB Human
Miller EL, Hargreaves DC, Kadoch C et al. TOP2 synergizes with BAF chromatin remodeling for both resolution and formation of facultative heterochromatin. Nat. Struct. Mol. Biol. 2017-02-27 [PMID: 28250416]
Staahl BT, Tang J, Wu W et al. Kinetic Analysis of npBAF to nBAF Switching Reveals Exchange of SS18 with CREST and Integration with Neural Developmental Pathways. J Neurosci 2013-06-19 [PMID: 23785148] (WB, ICC/IF, Human, Mouse) WB, ICC/IF Human, Mouse
Bao X, Tang J, Lopez-Pajares V et al. ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4. Cell Stem Cell 2013-02-07 [PMID: 23395444] (WB, IF/IHC, ICC/IF, Human) WB, IF/IHC, ICC/IF Human
Malik S, Oshima M, Roy N et al. SMARCA4 supports the oncogenic landscape of KRAS-driven lung tumors bioRxiv 2020-01-01 (WB, Human, Mouse) WB Human, Mouse
Li RZ, Li YY, Qin H, Li SS ACTL6A Promotes the Proliferation of Esophageal Squamous Cell Carcinoma Cells and Correlates with Poor Clinical Outcomes OncoTargets and therapy 2021-01-13 [PMID: 33469301] (WB, IF/IHC, Human) WB, IF/IHC Human
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ACTL6A
Entrez
Uniprot