PHF10 Antibody

Images

 
Western Blot: PHF10 Antibody [NBP2-19795] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF10 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PHF10 Antibody [NBP2-19795] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHF10 protein stained by PHF10 antibody diluted at 1:500. Red: Phalloidin, a ...read more
Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Mouse lung. PHF10 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: PHF10 Antibody [NBP2-19795] - 50 ug mouse colon lysate/extract 10% SDS-PAGE PHF10 antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Hela xenograft, using PHF10 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Rat lung. PHF10 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PHF10 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PHF10. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHF10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-19795 in the following applications:

Reactivity Notes

Bovine (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for PHF10 Antibody

  • BAF45a
  • BAF45APHD zinc finger protein XAP135
  • BRG1-associated factor 45a
  • FLJ10975
  • MGC111009
  • PHD finger protein 10
  • XAP135BRG1-associated factor, 45-KD, A

Background

This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PHF10 Antibody (NBP2-19795) (0)

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Review for PHF10 Antibody (NBP2-19795) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-19795:
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Images Ratings Applications Species Date Details
Western Blot PHF10 NBP2-19795
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4
reviewed by:
Xiaofeng Wang
WB Human 08/07/2014
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Summary

ApplicationWestern Blot
Sample TestedBT16 Nuclear Extract
SpeciesHuman
Lot40765

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FAQs for PHF10 Antibody (NBP2-19795) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

4
5
0
4
1
3
0
2
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1
0

Xiaofeng Wang
08/07/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PHF10
Uniprot