Activin RIB/ALK-4 Antibody

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Activin RIB/ALK-4 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Activin RIB/ALK‑4
  Leu32-Glu126
  Accession # Q61271
• Specificity: Detects mouse Activin RIB/ALK‑4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant human (rh) Activin RIB is observed, 5% cross‑reactivity with rhActivin R1A is observed and less than 1% cross‑reactivity with rhActivin RIIA and rhActivin RIIB is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACVR1B
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin RIB/ALK-4 Antibody

• activin A receptor, type IB
• Activin receptor type IB
• Activin receptor-like kinase 4
• Activin RIB
• ActivinRIB
• ACTRIB
• ACVR1B
• ACVRLK4ACTR-IB
• ALK4
• ALK-4
• ALK4activin A receptor, type II-like kinase 4
• EC 2.7.11
• EC 2.7.11.30
• Serine/threonine-protein kinase receptor R2
• SKR2activin receptor type-1B

Background

Activin RIB, also known as ALK-4, is a glycosylated 58 kDa type I transmembrane receptor that belongs to the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with, and phosphorylates, the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature mouse Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, mouse Activin RIB shares 93% and 98% aa sequence identity with human and rat Activin RIB, respectively. It shares 23%‑38% aa sequence identity with other mouse type I receptors Activin RIA, BMPR-IA, BMPR-IB, and TGF-beta RI. Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (5‑7). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (8). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation, apoptosis, and tumorigenesis (1, 9, 10).

1. Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
2. Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
3. Tsuchida, K. et al. (1995) Endocrinology 136:5493.
4. Ebner, R. et al. (1993) Science 260:1344.
5. Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
6. Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
7. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
8. Lewis, K.A. et al. (2000) Nature 404:411.
9. Gu, Z. et al. (1998) Genes Dev. 12:844.
10. Chen, Y. et al. (2004) Dev. Biol. 268:280.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Activin RIB/ALK-4 Antibody (AF1477)(2)

Publications using AF1477

• Veronique E. Miron, Amanda Boyd, Jing-Wei Zhao, Tracy J. Yuen, Julia M. Ruckh, Jennifer L. Shadrach et al. M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination Nature Neuroscience 9/1/2013 [PMID: 23872599]
• Bristol-Gould SK, Kreeger PK, Selkirk CG, Kilen SM, Cook RW, Kipp JL, Shea LD, Mayo KE, Woodruff TK Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool. Dev. Biol., 2006-06-17;298(1):132-48. 2006-06-17 [PMID: 16930587] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: ACVR1B
• Uniprot:
  • Q61271()