Activin A Antibody

Images

 
Western Blot: Activin A Antibody [NBP1-30928] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Inhibin beta A antibody diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Activin A Antibody [NBP1-30928] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Activin A Antibody [NBP1-30928] - CLEAR CELL OVCA xenograft, using Inhibin beta A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Biological Strategies: Western Blot: Activin A Antibody [NBP1-30928] - Western blot analyses of YY1 and inhibin betaA in MDA-231 cells co-transfected with miR-218 mimic and YY1-overexpressing plasmid, or the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Fe, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

Activin A Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Inhibin beta A. The exact sequence is proprietary.
Localization
Secreted
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Feline (100%), Chicken (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
INHBA
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-30928 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Activin A Antibody

  • Activin A
  • activin AB alpha polypeptide
  • Activin beta-A chain
  • erythroid differentiation factor
  • Erythroid differentiation protein
  • follicle-stimulating hormone-releasing protein
  • FSH-releasing protein
  • inhibin beta A chain
  • inhibin beta A subunit
  • Inhibin, beta-1

Background

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Activin A Antibody (NBP1-30928)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Activin A.

Transforming Growth Factor beta Signaling in Stem Cells
Transforming growth factor beta (TGF beta) signaling along with its family members have been implicated in development and maintenance of various organs. Stem cells are important contributors to this process and are characterized by their ability to s...  Read full blog post.

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Bioinformatics

Gene Symbol INHBA