Actin Gamma 1 Antibody (1G6) - Azide and BSA Free

Proximity Ligation Assay: Actin Gamma 1 Antibody (1G6) [H00000071-M01] - Analysis of protein-protein interactions between VASP and ACTG1. HeLa cells were stained with anti-VASP rabbit purified polyclonal 1:1200 and anti-ACTG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

ELISA: Actin Gamma 1 Antibody (1G6) [H00000071-M01] - Detection limit for recombinant GST tagged ACTG1 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func
• Clone: 1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Actin Gamma 1 Antibody (1G6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ACTG1 (AAH53572, 1 a.a. ~ 375 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
• Specificity: ACTG1 - actin, gamma 1 (1G6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTG1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Actin Gamma 1 Antibody (1G6) - Azide and BSA Free

• ACT
• ACTB
• ACTG
• actin, cytoplasmic 2
• actin, gamma 1
• cytoskeletal gamma-actin
• deafness, autosomal dominant 20; deafness, autosomal dominant 26
• DFNA20
• DFNA26
• Gamma-actin

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Actin Gamma 1 Antibody (H00000071-M01)(1)

Publication using H00000071-M01

• Yang HY, Kwon J, Choi HI et al. In-depth analysis of cysteine oxidation by the RBC proteome: Advantage of peroxiredoxin II knockout mice. Proteomics. 2011-11-23 [PMID: 22113967]

Bioinformatics

• Gene Symbol: ACTG1
• Entrez:
  • 71(Human)
• Uniprot:
  • AAH53572(Human)