ACSL3 Antibody - Azide and BSA Free

Western Blot: ACSL3 Antibody [H00002181-B01P] - Western Blot analysis of ACSL3 expression in transfected 293T cell line by ACSL3 MaxPab polyclonal antibody. Lane 1: ACSL3 transfected lysate (79. 2 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: ACSL3 Antibody [H00002181-B01P] - Analysis of purified antibody to ACSL3 on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ACSL3 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: ACSL3 (NP_004448.2, 1 a.a. - 720 a.a.) full-length human protein. MNNHVSSKPSTMKLKHTINPILLYFIHFLISLYTILTYIPFYFFSESRQEKSNRIKAKPVNSKPDSAYRSVNSLDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQKPKTNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPSDIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQISKGRNTPLCDSFVFRKVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGFVVPNQKELTELARKKGLKGTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIERMYGRK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: ACSL3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ACSL3 Antibody - Azide and BSA Free

• ACS3EC 6.2.1.3
• acyl-CoA synthetase long-chain family member 3
• FACL3lignoceroyl-CoA synthase
• fatty-acid-Coenzyme A ligase, long-chain 3
• LACS 3
• LACS3
• Long-chain acyl-CoA synthetase 3
• long-chain-fatty-acid--CoA ligase 3
• PRO2194

Background

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACSL3 Antibody (H00002181-B01P)(7)

Publications using H00002181-B01P

• Soltysik K, Ohsaki Y, Tatematsu T et al. Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner The Journal of cell biology 2021-01-04 [PMID: 33315072]
• Jung H, Shimizu-Albergine M, Shen X et al. TNF-? induces acyl-CoA synthetase 3 to promote lipid droplet formation in human endothelial cells. J Lipid Res. 2019-11-13 [PMID: 31722970]
• Salo V, Li S, Vihinen H et al. Seipin Facilitates Triglyceride Flow to Lipid Droplet and Counteracts Droplet Ripening via Endoplasmic Reticulum Contact. Dev Cell. 2019-06-06 [PMID: 31178403]
• Datta S, Liu Y, Hariri H et al. Cerebellar ataxia disease-associated Snx14 promotes lipid droplet growth at ER-droplet contacts J. Cell Biol. 2019-02-14 [PMID: 30765438] (ICC/IF, Human)
• Poppelreuther M, Sander S, Minden F et al. The metabolic capacity of lipid droplet localized acyl-CoA synthetase 3 is not sufficient to support local triglyceride synthesis independent of the endoplasmic reticulum in A431 cells. Biochim Biophys Acta Mol Cell Biol Lipids 2018-06-01 [PMID: 29526665] (Human)
• Kassan A, Herms A, Fernandez-Vidal A et al. Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains. J Cell Biol. 2013-12-23 [PMID: 24368806]
• Weedon-Fekjaer MS, Dalen KT, Solaas K et al. Activation of LXR increases acyl-CoA synthetase activity through direct regulation of ACSL3 in human placental trophoblast cells. [PMID: 20219900]

Bioinformatics

• Gene Symbol: ACSL3
• Uniprot:
  • NP_004448.2(Human)