ACSL1 Antibody (HL1530) - Azide and BSA Free

Western Blot: ACSL1 Antibody (HL1530) - Azide and BSA Free [NBP3-25351] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL1 antibody [HL1530] (NBP3-25351) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ACSL1 Antibody (HL1530) - Azide and BSA Free [NBP3-25351] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL1 antibody [HL1530] (NBP3-25351) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ACSL1 Antibody (HL1530) - Azide and BSA Free [NBP3-25351] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL1 antibody [HL1530] (NBP3-25351) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ACSL1 Antibody (HL1530) - Azide and BSA Free [NBP3-25351] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL1 antibody [HL1530] (NBP3-25351) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: HL1530
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ACSL1 Antibody (HL1530) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ACSL1. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACSL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for ACSL1 Antibody (HL1530) - Azide and BSA Free

• ACS1LACS 2
• Acyl-CoA synthetase 1
• acyl-CoA synthetase long-chain family member 1
• EC 6.2.1
• FACL1EC 6.2.1.3
• fatty-acid-Coenzyme A ligase, long-chain 1
• LACS 1
• LACSlong-chain 2
• lignoceroyl-CoA synthase
• Long-chain acyl-CoA synthetase 1
• Long-chain acyl-CoA synthetase 2
• Long-chain fatty acid-CoA ligase 2
• long-chain fatty-acid-coenzyme A ligase 1
• long-chain-fatty-acid--CoA ligase 1
• Palmitoyl-CoA ligase 1
• Palmitoyl-CoA ligase 2
• paltimoyl-CoA ligase 1

Background

Long-chain-fatty-acid--CoA ligase 1, or ACSL1, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in lipid biosynthesis and fatty acid degradation. Like other isozymes of this family, ALCS1 converts free long-chain fatty acids into fatty acyl-CoA esters. ACSL1 preferentially uses palmitoleate, oleate and linoleate, and altered expression of ACSL1 is thought to increase accumulation of triglycerides in the liver.

ALCS1 is strongly expressed in the heart, kindey, liver, skeletal muscle, and erythroid cells, and to a lesser extent in lung, brain, placenta and pancreas. This protein is predominantly expressed during the early stages of erythroid development, and expression is weak in reticulocytes and young erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACSL1