Acrosin Antibody (ACR-2) - BSA Free

Immunocytochemistry/Immunofluorescence: Acrosin Antibody (ACR-2) [NB500-419] - Staining of capacitated boar sperm. Acrosome visualized using mouse monoclonal ACR-2 (purified; secondary antibody labeled with FITC, green), DNA stained with DAPI (blue).

• Reactivity: Po, Bv(-), Ca(-), Hu(-)
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: ACR-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Acrosin Antibody (ACR-2) - BSA Free Summary

• Immunogen: Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
• Specificity: The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular) 1-12 ug/ml
  • Flow Cytometry 1-12 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
• Application Notes: Flow Cytometry: described in Ded et al., 2010 Immunocytochemistry: Membrane permeabilization (acetone) is essential. This antibody is CyTOF ready.
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Acrosin Antibody (ACR-2) - BSA Free

• acrosin
• ACRS
• preproacrosin
• proacrosin

Background

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACR