Acrosin Antibody (ACR-2)

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Product Details

Summary
Reactivity Po, Bv(-), Ca(-), Hu(-)Species Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
ACR-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Acrosin Antibody (ACR-2) Summary

Immunogen
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Specificity
The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACR
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • CyTOF-ready
Application Notes
Flow Cytometry: described in Ded et al., 2010 Immunocytochemistry: Membrane permeabilization (acetone) is essential.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Acrosin Antibody (ACR-2)

  • acrosin
  • ACRS
  • preproacrosin
  • proacrosin

Background

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Acrosin Antibody (NB500-419) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-419AF405
Alexa Fluor 488 Labeled NB500-419AF488
Alexa Fluor 647 Labeled NB500-419AF647
Alexa Fluor 700 Labeled NB500-419AF700
Allophycocyanin Labeled NB500-419APC
DyLight 350 Labeled NB500-419UV
DyLight 405 Labeled NB500-419V
DyLight 488 Labeled NB500-419G
DyLight 680 Labeled NB500-419FR
DyLight 755 Labeled NB500-419IR
PE Labeled NB500-419PE

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Bioinformatics

Gene Symbol ACR