Aconitase 2 Antibody

Images

 
Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-52015] - (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Aconitase 2 (aa541-555) in Heart lysate (35ug protein in RIPA buffer) using this antibody at 0.01ug/ml. Primary incubation was 1 hour. Detected by ...read more
Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Human (A), Mouse (B) and Rat (C) Adipose lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Aconitase 2 Antibody Summary

Immunogen
Peptide with sequence C-QDTYQHPPKDSSGQH corresponding to internal region according to NP_001089.1.
Epitope
C-QDTYQHPPKDSSGQH
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 90 kDa band observed in human heart lysates, in human, mouse and rat adipose lysates and in mouse, rat and porcine skeletal muscle lysates (calculated MW of 85.4 kDa band according to NP_001089.1). IHC-P: Human breast shows mitochondrial staining in the epithelial cells of the ducts.
Publications
Read Publication using NBP1-52015.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Aconitase 2 Antibody

  • aconitase 2, mitochondrial
  • Aconitase
  • aconitate hydratase, mitochondrial
  • ACONM
  • Citrate hydro-lyase
  • EC 4.2.1
  • EC 4.2.1.3
  • MGC20605
  • MGC33908

Background

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aconitase 2 Antibody (NBP1-52015)(1)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACO2
Entrez
Uniprot