Acetylcholinesterase/ACHE Antibody (HR2)

Images

 
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in U251 Cells. ...read more
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Cerebellum tissue.
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in Hela Cells. ...read more
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in Neuro-2a ...read more
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Rectum tissue.
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Brain tissue.

Product Details

Summary
Reactivity Hu, Bv, Fe, Rb, Mu(-), Rt(-)Species Glossary
Applications ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
HR2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Acetylcholinesterase/ACHE Antibody (HR2) Summary

Immunogen
Purified human cerebellar acetylcholinesterase.
Localization
Cell Membrane and Secreted
Marker
Early Neuronal Development Marker
Specificity
This antibody does not detect butyrylcholinesterase (BChE).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ACHE
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
IHC: Staining of AChE in human brain samples results in staining of nerve fibers and terminals. Cannot be used in WB to detect AChE. Not suitable for Flow.
Control
Acetylcholinesterase/ACHE Overexpression Lysate
Publications
Read Publication using NB300-528.

Reactivity Notes

No cross-reactivity with Frog.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Acetylcholinesterase/ACHE Antibody (HR2)

  • acetylcholinesterase (Yt blood group)
  • Acetylcholinesterase
  • ACHE
  • apoptosis-related acetylcholinesterase
  • ARACHE
  • EC 3.1.1
  • EC 3.1.1.7
  • N-ACHE
  • Yt blood group
  • YT

Background

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Acetylcholinesterase/ACHE Antibody (NB300-528)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for Acetylcholinesterase/ACHE Antibody (NB300-528) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACHE
Entrez