Acetylcholinesterase/ACHE Antibody (HR2) [CoraFluor™ 1]

• Reactivity: Hu, Bv, Fe, Rb, Mu(-), Rt(-)
• Applications: ELISA, ICC/IF, IHC, IP
• Clone: HR2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Acetylcholinesterase/ACHE Antibody (HR2) [CoraFluor™ 1] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Purified human cerebellar acetylcholinesterase.
• Localization: Cell Membrane and Secreted
• Marker: Early Neuronal Development Marker
• Specificity: This antibody does not detect butyrylcholinesterase (BChE).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACHE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB300-528. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

No cross-reactivity with Frog.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Acetylcholinesterase/ACHE Antibody (HR2) [CoraFluor™ 1]

• acetylcholinesterase (Yt blood group)
• Acetylcholinesterase
• ACHE
• apoptosis-related acetylcholinesterase
• ARACHE
• EC 3.1.1
• EC 3.1.1.7
• N-ACHE
• Yt blood group
• YT

Background

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACHE