Acetylcholinesterase/ACHE Antibody (HR2) [mFluor Violet 610 SE]

• Reactivity: Hu, Bv, Fe, Rb, Mu(-), Rt(-)
• Applications: ELISA, ICC/IF, IHC, IP
• Clone: HR2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 610 SE

Acetylcholinesterase/ACHE Antibody (HR2) [mFluor Violet 610 SE] Summary

• Immunogen: Purified human cerebellar acetylcholinesterase.
• Localization: Cell Membrane and Secreted
• Marker: Early Neuronal Development Marker
• Specificity: This antibody does not detect butyrylcholinesterase (BChE).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACHE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

No cross-reactivity with Frog.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Acetylcholinesterase/ACHE Antibody (HR2) [mFluor Violet 610 SE]

• acetylcholinesterase (Yt blood group)
• Acetylcholinesterase
• ACHE
• apoptosis-related acetylcholinesterase
• ARACHE
• EC 3.1.1
• EC 3.1.1.7
• N-ACHE
• Yt blood group
• YT

Background

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACHE