Acetyl-CoA Carboxylase Activity Assay Kit (Colorimetric)

Acetyl-CoA Carboxylase Activity Assay Kit (Colorimetric) [NBP3-24454] - Standard Reference Curve

Acetyl-CoA Carboxylase Activity Assay Kit (Colorimetric) [NBP3-24454] - Assay Example

• Reactivity: All-NA
• Applications: Func
• Suitable Sample Type: Animal tissue and plant tissue
• Standard Curve Range: 6.78 - 138 U/L
• Sensitivity: 6.78 U/L

Acetyl-CoA Carboxylase Activity Assay Kit (Colorimetric) Summary

• Description: Detection instrument: Microplate reader (650 - 670 nm, optimum wavelength: 660 nm)
  Assay time: 70 min
• Standard Curve Range: 6.78 - 138 U/L
• Sensitivity: 6.78 U/L
• Inter-Assay: %CV < 8
• Intra-Assay: %CV < 4
• Spike Recovery: Average recovery rate: 98%
• Kit Type: Activity Assay Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Acetyl-CoA Carboxylase Activity Assay Kit (Colorimetric)

• ACAC
• Acac1
• ACACAD
• ACC
• ACC1
• ACCA
• ACC-alpha
• ACCalphaACACalpha
• acetyl-CoA carboxylase 1
• acetyl-Coenzyme A carboxylase alpha
• hACC1

Background

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.