Acetyl-CoA Carboxylase alpha/ACACA Antibody

Product Discontinued

Acetyl-CoA Carboxylase alpha/ACACA Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human ACCalpha.
• Specificity: This ACC alpha (K86) polyclonal antibody is expected to detect the endogenous levels of ACC alpha protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACACA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 265 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Acetyl-CoA Carboxylase alpha/ACACA Antibody

• ACACA
• ACACacetyl-CoA carboxylase 1
• ACC1
• ACC1ACC
• ACCA
• ACC-alpha
• AcetylCoA Carboxylase alpha
• Acetyl-CoA Carboxylase alpha
• acetyl-Coenzyme A carboxylase alpha
• EC 6.4.1.2

Background

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl- CoA carboxylase in human muscle. ACCalpha (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCbeta (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACCalpha is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme. The gene encoding ACCalpha, which maps to human chromosome 17, encodes the 265 kDa alpha form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACCbeta is homologous to that of ACCalpha except for an additional peptide of about 150 amino acids at the N-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-92011)(1)

Publication using NB100-92011

• Dihingia A, Bordoloi J, Dutta P et al. Hexane-Isopropanolic Extract of Tungrymbai, a North-East Indian fermented soybean food prevents hepatic steatosis via regulating AMPk-mediated SREBP/FAS/ACC/HMGCR and PPARa/CPT1A/UCP2 pathways. Sci Rep. 2018-07-03 [PMID: 29968750] (WB, Mouse)

Bioinformatics

• Gene Symbol: ACACA
• Entrez:
  • 31(Human)
  • 107476(Mouse)
  • 60581(Rat)
• Uniprot:
  • Q13085()