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ACE/CD143 Antibody (9B9)

Images

 
Formalin-fixed, paraffin-embedded human small intestine stained with ACE/CD143 antibody (9B9). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
9B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

ACE/CD143 Antibody (9B9) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08316)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Additional Information
Clone 9B9 was used by HLDA to establish CD designation.
Immunogen
Full-length recombinant human ACE/CD143 protein (Uniprot: P12821)
Localization
Cell Surface
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACE
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for ACE/CD143 Antibody (9B9)

  • ACE
  • ACE1angiotensin converting enzyme, somatic isoform
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
  • carboxycathepsin
  • CD143 antigen
  • CD143
  • DCP
  • DCP1
  • DCP1angiotensin-converting enzyme
  • dipeptidyl carboxypeptidase 1
  • Dipeptidyl carboxypeptidase I
  • EC 3.2.1.-
  • EC 3.4.15.1
  • Kininase II
  • MGC26566
  • MVCD3
  • peptidase P
  • testicular ECA

Background

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ACE
Uniprot