Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse ACE/CD143 Monoclonal Antibody (Catalog # MAB1513) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody ...read more
angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
dipeptidyl carboxypeptidase 1
Dipeptidyl carboxypeptidase I
(also known as peptidyl-dipetidase A) is a zinc metallopeptidase
important for blood pressure control and water and salt metabolism (1).
It cleaves the C-terminal dipeptide from angiotensin I to produce the
potent vasopressor octapeptide angiotensin II and inactivates bradykinin
by the sequential removal of two C-terminal dipeptides. In addition to
the two physiological substrates, ACE cleaves C-terminal dipeptides from
various oligopeptides with a free C-terminus. Because of its location
and specificity, ACE plays additional roles in immunity, reproduction
and neuropeptide regulation. For example, ACE degrades Alzheimer amyloid
beta -peptide (A beta ), retards A beta aggregation, deposition, fibril formation,
and inhibits cytotoxicity (2). ACE is a type I membrane protein and
exists in two isoforms (1). Somatic ACE, found in endothelial,
epithelial and neuronal cells, comprises two highly similar catalytic
domains called N- and C-domains. Germinal ACE, found exclusively in the
testes, comprises a single catalytic domain identical to the C-domain of
somatic ACE except for an N-terminal 67 residue germinal ACE-specific
sequence. Physiological functions of the two tissue-specific isozymes
are not interchangeable (3). For example, sperm-specific expression of
the germinal ACE, not the somatic ACE, in ACE knockout male mice
restored fertility. Soluble ACE is present in many biological fluids,
such as serum, seminal fluid, amniotic fluid and cerebrospinal fluid
(1). The soluble ACE is derived from the membrane forms by actions of
secretases or sheddases. The identities of the secretases have not been
revealed, although they belong to the family of zinc metallopeptidases
Corvol, P. et al. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.) p. 332, Academic Press, San Diego.
Hu, J. et al. (2001) J. Biol. Chem. 276:47863.
Kessler, S.P. et al. (2000) J. Biol. Chem. 275:26259.
Eyries, M. et al. (2001) J. Biol. Chem. 276:5525.
Alfalah, M. et al. (2001) J. Biol. Chem. 276:21105.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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