ABCG8 Antibody (1B10A5) - Azide and BSA Free

Western Blot: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were incubated with anti-ABCG8 Antibody (1B10A5) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Immunohistochemistry: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560] - Immunohistochemical analysis of ABCG8 in mouse intestine using NBP1-71706. Image from the standard format of this antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP, KO
• Clone: 1B10A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

ABCG8 Antibody (1B10A5) - Azide and BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse ABCG8 Antibody (1B10A5) - Azide and BSA Free (NBP1-71706) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length mouse ABCG8 [Swiss-Prot# Q9DBM0]
• Localization: Membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCG8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 25ug
  • Knockout Validated
  • Western Blot 1:1000
• Application Notes: This ABCG8 (clone 1B10A5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ABCG8 Antibody (1B10A5) - Azide and BSA Free

• ATP-binding cassette sub-family G member 8
• ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
• ATP-binding cassette, sub-family G (WHITE), member 8
• GBD4ATP-binding cassette, subfamily G, member 8
• MGC142217
• sterolin 2
• sterolin-2
• STSL

Background

Normal digestion is partially facilitated by the function of ABCG8 and its counterpart ABCG5, which together form a heterodimer which transports cholesterol out of the liver and into bile. Subsequently, cholesterol is eliminated from the bodies of healthy individuals. However, in cases of insulin resistance, this functionality is impaired, and digestion does not result in complete elimination of harmful cholesterol. Unsurprisingly, mutations in ABCG8 can lead to gallstone disease, biliary cancer, hypercholesterolemia and atherosclerosis. In studies related to diabetes, heart disease, and digestive cancers, ABCG8 is commonly assessed for normal expression, function and interaction with ABCG5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABCG8