5'-Nucleotidase/CD73 Antibody (606112)

Human peripheral blood lymphocytes were labeled with Mouse Anti-Human/Equine 5'‑Nucleotidase/CD73 Mono­clonal Antibody (Catalog # MAB5795) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Equine 5'‑Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB5795, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu, Eq
• Applications: Flow, CyTOF-ready
• Clone: 606112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

5'-Nucleotidase/CD73 Antibody (606112) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human 5'‑Nucleotidase/CD73
  Trp27-Ile511
  Accession # P21589
• Specificity: Detects recombinant human 5'-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5'-Nucleotidase is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NT5E
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5'-Nucleotidase/CD73 Antibody (606112)

• 5' nucleotidase (CD73)
• 5'-NT
• 5-NT
• 5'-Nucleotidase
• 5'-nucleotidase, ecto (CD73)
• CD73 antigen
• CD73
• E5NT
• EC 3.1.3.5
• Ecto-5'-nucleotidase
• eN
• eNT
• NT
• NT55'-nucleotidase
• NT5E
• NTE
• Purine 5-Prime-Nucleotidase

Background

5'-Nucleotidase (also [ecto]-5'-nucleotidase/5'-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5'-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3⁺ Treg lymphocytes, FDCs and B cells. 5'-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5'-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5'‑Nucleotidase shares 88% aa identity with both mouse and rat 5'-Nucleotidase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5'-Nucleotidase/CD73 Antibody (MAB5795)(1)

Publication using MAB5795

• F Muhammad, D Wang, A Montieth, S Lee, J Preble, CS Foster, TA Larson, K Ding, JD Dvorak, DJ Lee PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease Sci Rep, 2019;9(1):16941. 2019 [PMID: 31729418] (Flow Cytometry, Human)

FAQs for 5'-Nucleotidase/CD73 Antibody (MAB5795). (Showing 1 - 1 of 1 FAQ).

1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: NT5E
• Uniprot:
  • P21589()