5-Lipoxygenase Antibody

Images

 
Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Detection of LOX5 in human peripheral leukocytes using NB110-58749 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).
Simple Western: 5-Lipoxygenase Antibody [NB110-58749] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RbSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

5-Lipoxygenase Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region within residues 600-674 of human 5-Lipoxygenase. [Swiss-Prot# P09917]
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALOX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Simple Western 10 ug/ml
Application Notes
This 5 Lipoxygenase antibody is useful for Western Blot, where a band is seen at approx. 80 kDa. There may be some non-specific bands at non-interfering lower molecular weights.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Blood Leukocyte Lysate (NB820-59174)
Control Peptide
5-Lipoxygenase Peptide (NB110-58749PEP)

Reactivity Notes

Human, rabbit, mouse, rat and bovine.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 5-Lipoxygenase Antibody

  • 5Lipoxygenase
  • 5-Lipoxygenase
  • 5-LO
  • 5-LOX
  • 5LPG
  • ALOX5
  • arachidonate 5-lipoxygenase
  • arachidonic 5-lipoxygenase alpha-10 isoform
  • arachidonic 5-lipoxygenase delta-10-13 isoform
  • arachidonic 5-lipoxygenase delta-13 isoform
  • arachidonic 5-lipoxygenase delta-p10 isoform
  • arachidonic acid 5-lipoxygenase
  • EC 1.13.11
  • EC 1.13.11.34
  • leukotriene A4 synthase
  • LOG5
  • LOX5A
  • MGC163204

Background

5-lipoxygenase (5LO) is a pro-inflammatory enzyme widely expressed in CNS where its level increases in an age-dependent manner and its activity is regulated by the presence/availability of FLAP (5LO activating protein) that form a functional complex with 5LO to catalyze arachidonic acid oxidation to produce lipid molecules with complex signaling properties such as leukotrienes. Reaction catalyzed by 5LO is considered as first step in the leukotriene biosynthesis and it is highly critical to inflammatory processes. Moreover, knockout of 5LO as well as FLAP has been shown to result in anxiety-like behavior in mice and 5LO is emerging as a new active player in Alzheimer's disease (AD) pathogenesis. 5LO levels are significantly increased in AD postmortem brain tissues and its genetic absence or pharmacological blockade both result in a significant reduction in the abeta levels as well as deposition in a transgenic mouse model of AD-like amyloidosis (i.e., Tg2576) mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for 5-Lipoxygenase Antibody (NB110-58749) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-58749AF405
Alexa Fluor 488 Labeled NB110-58749AF488
Alexa Fluor 647 Labeled NB110-58749AF647
Alexa Fluor 700 Labeled NB110-58749AF700
Biotin Labeled NB110-58749B
DyLight 405 Labeled NB110-58749V
DyLight 488 Labeled NB110-58749G
DyLight 550 Labeled NB110-58749R
DyLight 650 Labeled NB110-58749C
HRP Labeled NB110-58749H

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Bioinformatics

Gene Symbol ALOX5
Entrez