5-Lipoxygenase Antibody - BSA Free

Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Western blotting data for TLR4, COX-2, IL-1beta, iNOS, 5-Lipoxygenase. Data are representative of three independent experiment. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2020.00142/full), licensed under a CC-BY license.

Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Detection of LOX5 in human peripheral leukocytes using NB110-58749 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).

Simple Western: 5-Lipoxygenase Antibody [NB110-58749] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Western blotting data for TLR4, COX-2, IL-1 beta , iNOS, 5-LOX. Data are representative of three independent experiment.

• Reactivity: Hu, Mu, Rt, Bv, Rb
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

5-Lipoxygenase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit 5-Lipoxygenase Antibody - BSA Free (NB110-58749) is a polyclonal antibody validated for use in WB and Simple Western. Anti-5-Lipoxygenase Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a C-terminal region within residues 600-674 of human 5-Lipoxygenase. [Swiss-Prot# P09917]
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALOX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/ml
  • Western Blot 1:500-1:1000
• Application Notes: This 5 Lipoxygenase antibody is useful for Western Blot, where a band is seen at approx. 80 kDa. There may be some non-specific bands at non-interfering lower molecular weights.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Lung lysate 0.5 mg/mL, separated by Size, antibody dilution of 10 ug/mL. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Blood Leukocyte Whole Cell Lysate

• Control Peptide:

  5-Lipoxygenase Peptide (NB110-58749PEP)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 5-Lipoxygenase Antibody - BSA Free

• 5Lipoxygenase
• 5-Lipoxygenase
• 5-LO
• 5-LOX
• 5LPG
• ALOX5
• arachidonate 5-lipoxygenase
• arachidonic 5-lipoxygenase alpha-10 isoform
• arachidonic 5-lipoxygenase delta-10-13 isoform
• arachidonic 5-lipoxygenase delta-13 isoform
• arachidonic 5-lipoxygenase delta-p10 isoform
• arachidonic acid 5-lipoxygenase
• EC 1.13.11
• EC 1.13.11.34
• leukotriene A4 synthase
• LOG5
• LOX5A
• MGC163204

Background

5-lipoxygenase (5LO) is a pro-inflammatory enzyme widely expressed in CNS where its level increases in an age-dependent manner and its activity is regulated by the presence/availability of FLAP (5LO activating protein) that form a functional complex with 5LO to catalyze arachidonic acid oxidation to produce lipid molecules with complex signaling properties such as leukotrienes. Reaction catalyzed by 5LO is considered as first step in the leukotriene biosynthesis and it is highly critical to inflammatory processes. Moreover, knockout of 5LO as well as FLAP has been shown to result in anxiety-like behavior in mice and 5LO is emerging as a new active player in Alzheimer's disease (AD) pathogenesis. 5LO levels are significantly increased in AD postmortem brain tissues and its genetic absence or pharmacological blockade both result in a significant reduction in the abeta levels as well as deposition in a transgenic mouse model of AD-like amyloidosis (i.e., Tg2576) mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-Lipoxygenase Antibody (NB110-58749)(1)

Publication using NB110-58749

• Di Federico M, Ancora M, Luciani M et al. Pro-Inflammatory Response of Bovine Polymorphonuclear Cells Induced by Mycoplasma mycoides subsp. mycoides Front Vet Sci 2020-03-27 [PMID: 32292794] (WB, Bovine)

Bioinformatics

• Gene Symbol: ALOX5