Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9)

Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Leukotriene A4 hydrolasemonoclonal antibody.

Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded prostate tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.

Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Leukotriene A4 hydrolase(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Leukotriene A4 hydrolase.

Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded endometrium tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded pancreas tissue using anti-Leukotriene A4 hydrolasemouse monoclonal antibody.

Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Immunoprecipitation of LTA4H by using TrueMab monoclonal anti-LTA4H antibody (Negative control: IP without adding anti-LTA4H antibody). For each experiment, 500ul of DDK tagged LTA4H overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant-LTA4H antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, IHC, IHC-P, IP
• Clone: OTI1E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.7 mg/ml

Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) Summary

• Immunogen: Full-length protein expressed in 293T cell transfected with human LTA4H expression vector
• Specificity: This antibody is specific for Homo sapiens leukotriene A4 hydrolase (LTA4H).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LTA4H
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2-4ug/mg
  • Western Blot 1:2000
• Theoretical MW: 69.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.7 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9)

• EC 3.3.2.6
• Leukotriene A(4) hydrolase
• Leukotriene A4 Hydrolase
• leukotriene A-4 hydrolase
• LTA-4 hydrolase
• LTA4
• LTA4H

Background

Hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leukotriene A4 Hydrolase/LTA4H Antibody (NBP1-47829)(1)

Publication using NBP1-47829

• Nordio L, Bazzocchi C, Genova F et al. Molecular and Immunohistochemical Expression of LTA4H and FXR1 in Canine Oral Melanoma Frontiers in veterinary science 2021-12-13 [PMID: 34966807] (IHC-P, Human)

Bioinformatics

• Gene Symbol: LTA4H
• Entrez:
  • 4048(Human)