4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

Images

 
Activated mouse splenocytes were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Goat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified ...read more
Resting mouse splenocytes were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Goat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal ...read more
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with recombinant mouse 4-1BB/Fc chimera. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse ...read more
4‑1BB/TNFRSF9/CD137 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) at 1.7 µg/mL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, Func
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

4-1BB/TNFRSF9/CD137 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse 4‑1BB/TNFRSF9/CD137
Val24-Leu187
Accession # P20334
Specificity
Detects mouse 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat 4-1BB/TNFRSF9 is observed. In Western blots, less than 5% cross-reactivity with recombinant human 4-1BB and recombinant mouse EDAR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF9
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • Agonist Activity
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 4
using
AF937 in the following applications:

Publications
Read Publications using
AF937 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

  • 4-1BB ligand receptor
  • 41BB
  • 4-1BB
  • CD137 antigen
  • CD137
  • CD137MGC2172
  • CDw137
  • FLJ43501
  • ILA
  • ILAhomolog of mouse 4-1BB
  • induced by lymphocyte activation (ILA)
  • interleukin-activated receptor, homolog of mouse Ly63
  • receptor protein 4-1BB
  • T cell antigen ILA
  • T-cell antigen 4-1BB homolog
  • T-cell antigen ILA
  • TNFRSF9
  • tumor necrosis factor receptor superfamily member 9
  • tumor necrosis factor receptor superfamily, member 9

Background

4-1BB, also known as CD137 and ILA (induced by lymphocyte activation), is a TNF receptor superfamily member and has been designated TNFRSF9. Mouse 4‑1BB cDNA encodes a 256 amino acid (aa) residues type I transmembrane protein with a putative 23 aa signal peptide, a 164 aa extracellular domain, a 21 aa transmembrane domain and a 48 aa cytoplasmic region (1‑3). A soluble 4‑1BB is released from surfaces of cells expressing the transmembrane protein (4).
Mouse 4‑1BB shares approximately 60% aa sequence identity with its human counterpart. 4‑1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils (5). The ligand for 4‑1BB (4‑1BBL), also named TNFSF9, belongs to the TNF ligand superfamily. 4‑1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. In response to 4‑1BBL binding, 4‑1BB transduce a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In vivo, the costimulatory activity of 4‑1BB has been shown to be important in graft-vs-host disease and antiviral CTL responses. On dendritic cells, 4‑1BB is a DC-activating molecules that enhances cytokine production and up‑regulates expression of B7-1 and B7-2 costimulatory molecules, resulting in an improved ability to stimulate T cell responses (1‑5).

  1. Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631.
  2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  3. Kwon, B.S. and S.M. Weissman (1989) Proc. Nat. Acad. Sci. USA 86:1963.
  4. Wilcox, R.A. et al. (2002) J. Immunol. 168:4262.
  5. Kwon, B., H.W. Lee and B.S. Kwon, 2002, TRENDS in Immunology 23:378.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 4-1BB/TNFRSF9/CD137 Antibody (AF937)(2)

Review for 4-1BB/TNFRSF9/CD137 Antibody (AF937) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF937:
Filter by Applications
IHC-P
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Mouse 01/12/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22799524
SpeciesMouse
Special ApplicationsSee PMID 22799524 for further details.

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Recent Reviews

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5
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4
1
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2
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1
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Anonymous
01/12/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol TNFRSF9
Uniprot