4-1BB Ligand/TNFSF9 Antibody (TKS-1) - Chimeric - Azide and BSA Free

ELISA: 4-1BB Ligand/TNFSF9 Antibody (TKS-1) [NBP2-81115] - ELISA of anti-4-1BBL antibody on 4-1BBL-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-4-1BBL antibody TKS-1 (NBP2-81115; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse 4-1BBL Fc-fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using NBP2-81115. For signal detection, a 1:4000 dilution of HRP-fused anti-rabbit IgG antibody was used.

• Reactivity: Mu
• Applications: ELISA, Flow, B/N
• Clone: TKS-1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

4-1BB Ligand/TNFSF9 Antibody (TKS-1) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Murine B-cell lymphoma 2PK-3 cells injected into SD rats.
• Specificity: This antibody binds to murine 4-1BB Ligand/TNFSF9 and competes with binding of 4-1BB.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TNFSF9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Flow Cytometry
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. This antibody has been used to detect 4-1BBL by flow cytometry. It is able to block 4-1BBL interacting with 4-1BB and prevent signalling.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for 4-1BB Ligand/TNFSF9 Antibody (TKS-1) - Chimeric - Azide and BSA Free

• 41BB Ligand
• 4-1BB Ligand
• 4-1BBL
• 4-1BB-L
• CD137L
• homolog of mouse 4-1BB-L
• receptor 4-1BB ligand
• TNFSF9
• tumor necrosis factor (ligand) superfamily, member 9
• tumor necrosis factor ligand superfamily member 9

Background

CD137 exists on the cell surface as a monomer with a molecular mass of 30 kDa and as a dimer of 55 kDa. Human and mouse CD137 share 60% amino acid identity. CD137 (4-1BB), a member of the tumor necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen over stimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF9