2B4/CD244/SLAMF4 Antibody (244F4)

Images

 
Flow Cytometry: 2B4/CD244/SLAMF4 Antibody (244F4) [NBP2-00223] - Analysis using the FITC conjugate of NBP2-00223. Staining of C57Bl/6 splenocytes with Anti-Mouse NK1.1 PE and 0.125 ug of Rat IgG2a K Isotype Control FITC ...read more
Flow Cytometry: 2B4/CD244/SLAMF4 Antibody (244F4) [NBP2-00223] - C57BL/6 splenocytes were cultured for 6 days in Mouse IL-2 Recombinant Protein and subsequently stained with 0.125 ug of Anti-Mouse CD244.2 (2B4) Purified ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow
Clone
244F4
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

2B4/CD244/SLAMF4 Antibody (244F4) Summary

Immunogen
The immunogen for this antibody is CD244.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD244
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
Application Notes
This 244F4 antibody has been tested by flow cytometric analysis of IL-2-stimulated mouse splenocytes. This can be used at less than or equal to 0.06 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 244F4 antibody has also been reported for use in immunoblotting (WB). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for 2B4/CD244/SLAMF4 Antibody (244F4)

  • 2B4
  • CD244 antigen
  • CD244 molecule, natural killer cell receptor 2B4
  • CD244
  • h2B4
  • NAIL
  • NAILNK cell activation-inducing ligand
  • natural killer cell receptor 2B4
  • NKR2B4
  • NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4
  • Nmrk
  • SLAMF4
  • SLAMF4NK cell activation inducing ligand NAIL

Background

Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non-MHC dependent cytotoxicity. Expression is found on natural killer (NK) cells and many NKT cells and CD8+ cells. In C57BL/6 mice, two splice variants exist that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner through signaling through SHP-2, while the short form functions in an activating manner. The ligand for CD244 is CD48 which is expressed on all hematopoietic cells. Recent data suggests that CD244 interactions with CD48, but not CD2 interaction with CD48, is essential for IL-2-driven expansion and activation of murine NK cells. Reports show CD244 expression can discriminate hematopoietic progenitors. Hematopoietic stem cells (HSC) are highly purified as CD150+CD244-CD48- cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244+CD150-CD48- and the most restricted progenitors are CD48+CD244+CD150-.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-00223AF405
Alexa Fluor 488 Labeled NBP2-00223AF488
Alexa Fluor 647 Labeled NBP2-00223AF647
Alexa Fluor 700 Labeled NBP2-00223AF700
Allophycocyanin Labeled NBP2-00223APC
DyLight 350 Labeled NBP2-00223UV
DyLight 405 Labeled NBP2-00223V
DyLight 488 Labeled NBP2-00223G
DyLight 680 Labeled NBP2-00223FR
DyLight 755 Labeled NBP2-00223IR
FITC Labeled NBP2-00221
PE Labeled NBP2-00223PE
PerCP Labeled NBP2-00223PCP

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Bioinformatics

Gene Symbol CD244
Entrez