2B4/CD244/SLAMF4 Antibodies

HAF017). A specific band was detected for 2B4/CD244/SLAMF4 at approximately 65 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for 2B4/CD244/SLAMF4 at approximately 65 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NK-RL human natural killer lymphocytic leukemia cell line, NK-92 human natural killer lymphoma cell line, and Daudi human Burkitt's lymphoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for 2B4/CD244/SLAMF4 at approximately 65 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

FAB2408A) and either (A) Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox"> FAB2408A) and either (A) Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="2B4/CD244/SLAMF4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human 2B4/CD244/SLAMF4 Monoclonal Antibody (Catalog # MAB1039) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

F0101B). Quadrant markers were set based on Mouse IgG2B isotype control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based on Mouse IgG2B isotype control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) Human 2B4/CD244/SLAMF4 and eGFP or (B) irrelevant protein, was stained with Mouse Anti-Human 2B4/CD244/SLAMF4 Monoclonal Antibody (Catalog # MAB10393) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B isotype control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB0041) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse anti-Human CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB0041) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse anti-Human CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

3644-CD) to immobilized Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera (Catalog # 1039-2B) coated at 5 ug/mL (100 µL/well). At 10 ug/mL, this antibody will block >90% of the binding." class="big_lightbox"> 3644-CD) to immobilized Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera (Catalog # 1039-2B) coated at 5 ug/mL (100 µL/well). At 10 ug/mL, this antibody will block >90% of the binding." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="2B4/CD244/SLAMF4 was detected in immersion fixed human natural killer cells using Mouse Anti-Human 2B4/CD244/SLAMF4 Monoclonal Antibody (Catalog # MAB10391) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />