2B4/CD244/SLAMF4 Antibody (244F4) [FITC]


Flow Cytometry: 2B4/CD244/SLAMF4 Antibody (244F4) [FITC] [NBP2-00221] - Staining of C57Bl/6 splenocytes with Anti-Mouse NK1.1 PE and 0.125 ug of Rat IgG2a K Isotype Control FITC (left) or 0.125 ug of Anti-Mouse CD244.2 ...read more

Product Details

Reactivity MuSpecies Glossary
Applications Flow
0.5 mg/ml

2B4/CD244/SLAMF4 Antibody (244F4) [FITC] Summary

The immunogen for this antibody is CD244.
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
FITC (A=495, E=519)
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Flow Cytometry 1:10-1:1000
Application Notes
This 244F4 antibody has been tested by flow cytometric analysis of IL-2-stimulated mouse splenocytes. This can be used at less than or equal to 0.06 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 244F4 antibody has also been reported for use in immunoblotting (WB). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes


Alternate Names for 2B4/CD244/SLAMF4 Antibody (244F4) [FITC]

  • CD244 antigen
  • CD244 molecule, natural killer cell receptor 2B4
  • CD244
  • h2B4
  • NAIL
  • NAILNK cell activation-inducing ligand
  • natural killer cell receptor 2B4
  • NKR2B4
  • NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4
  • Nmrk
  • SLAMF4
  • SLAMF4NK cell activation inducing ligand NAIL


Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non-MHC dependent cytotoxicity. Expression is found on natural killer (NK) cells and many NKT cells and CD8+ cells. In C57BL/6 mice, two splice variants exist that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner through signaling through SHP-2, while the short form functions in an activating manner. The ligand for CD244 is CD48 which is expressed on all hematopoietic cells. Recent data suggests that CD244 interactions with CD48, but not CD2 interaction with CD48, is essential for IL-2-driven expansion and activation of murine NK cells. Reports show CD244 expression can discriminate hematopoietic progenitors. Hematopoietic stem cells (HSC) are highly purified as CD150+CD244-CD48- cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244+CD150-CD48- and the most restricted progenitors are CD48+CD244+CD150-.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, S-ELISA
Species: Mu
Applications: Flow, ELISA(Cap), ELISA(Det), ELISA(Sta)
Species: Hu
Applications: WB, Flow, CostimT, Neut
Species: Hu, Mu
Applications: WB, Simple Western
Species: Hu
Applications: WB, Flow, AgAct, ICC
Species: Hu
Applications: WB, B/N, Flow, IHC, IHC-Fr
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
Species: Hu
Applications: WB
Species: Hu
Applications: Flow, Block

Publications for 2B4/CD244/SLAMF4 Antibody (NBP2-00221) (0)

There are no publications for 2B4/CD244/SLAMF4 Antibody (NBP2-00221).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 2B4/CD244/SLAMF4 Antibody (NBP2-00221) (0)

There are no reviews for 2B4/CD244/SLAMF4 Antibody (NBP2-00221). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for 2B4/CD244/SLAMF4 Antibody (NBP2-00221) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-00223APC
DyLight 350 Labeled NBP2-00223UV
DyLight 405 Labeled NBP2-00223V
DyLight 405LS Labeled NBP2-00223V3
DyLight 488 Labeled NBP2-00223G
DyLight 680 Labeled NBP2-00223FR
DyLight 755 Labeled NBP2-00223IR
PE Labeled NBP2-00223PE
PerCP Labeled NBP2-00223PCP

Additional 2B4/CD244/SLAMF4 Products

Related Products by Gene

Bioinformatics Tool for 2B4/CD244/SLAMF4 Antibody (NBP2-00221)

Discover related pathways, diseases and genes to 2B4/CD244/SLAMF4 Antibody (NBP2-00221). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 2B4/CD244/SLAMF4 Antibody (NBP2-00221)

Discover more about diseases related to 2B4/CD244/SLAMF4 Antibody (NBP2-00221).

Pathways for 2B4/CD244/SLAMF4 Antibody (NBP2-00221)

View related products by pathway.

PTMs for 2B4/CD244/SLAMF4 Antibody (NBP2-00221)

Learn more about PTMs related to 2B4/CD244/SLAMF4 Antibody (NBP2-00221).

Blogs on 2B4/CD244/SLAMF4

There are no specific blogs for 2B4/CD244/SLAMF4, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol CD244

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-00221 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

Human IL-2 Protein