17 beta-HSD1/HSD17B1 Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

17 beta-HSD1/HSD17B1 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human 17 β-HSD1/HSD17B1
  Ala2-Gln328
  Accession # P14061
• Specificity: Detects human 17 beta -HSD1/HSD17B1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 17 beta-HSD1/HSD17B1 Antibody [Alexa Fluor® 532]

• 17 betaHSD1
• 17 beta-HSD1
• 17-beta-HSD 1
• 17-beta-hydroxysteroid dehydrogenase type 1
• 20 alpha-hydroxysteroid dehydrogenase
• 20-alpha-HSD
• E17KSR
• E2DH
• EC 1.1.1.62
• EDH17B1
• EDH17B2
• EDH17B2EDHB17
• EDHB17
• estradiol 17-beta-dehydrogenase 1
• estradiol 17-beta-dehydrogenase-1
• HSD17
• HSD17B1
• hydroxysteroid (17-beta) dehydrogenase 1 isoform
• hydroxysteroid (17-beta) dehydrogenase 1
• MGC138140
• Placental 17-beta-hydroxysteroid dehydrogenase
• SDR28C1
• short chain dehydrogenase/reductase family 28CE, member 1

Background

HSD17B1 (Hydroxysteroid 17-beta dehydrogenase type 1; also 17  beta ‑HSD1 and E2DH) is a 33-34 kDa member of the short chain dehydrogenase/reductase (SDR) superfamily of molecules. It is a cytoplasmic breast, placental and gonadal enzyme that both converts DHEA to estrogen A-diol, and inactivates DHT through oxidation and reduction. Human HSD17B1 is 328 amino acids (aa) in length. It contains an NADP binding site (aa 10-38) and a steroid catalytic site (aa 210-221). HSD17B1 functions as a 68 kDa noncovalently-linked homodimer. Over aa 2-328, human HSD17B1 shares 65% aa identity with mouse HSD17B1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.