17 beta-HSD1/HSD17B1 Antibody (860020) [Allophycocyanin]

• Reactivity: Hu
• Applications: WB
• Clone: 860020
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

17 beta-HSD1/HSD17B1 Antibody (860020) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human 17 beta ‑HSD1/HSD17B1
  Ala2-Gln328
  Accession # P14061
• Specificity: Detects human 17 beta ‑HSD1/HSD17B1 in ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSD17B1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for 17 beta-HSD1/HSD17B1 Antibody (860020) [Allophycocyanin]

• 17 betaHSD1
• 17 beta-HSD1
• 17-beta-HSD 1
• 17-beta-hydroxysteroid dehydrogenase type 1
• 20 alpha-hydroxysteroid dehydrogenase
• 20-alpha-HSD
• E17KSR
• E2DH
• EC 1.1.1.62
• EDH17B1
• EDH17B2
• EDH17B2EDHB17
• EDHB17
• estradiol 17-beta-dehydrogenase 1
• estradiol 17-beta-dehydrogenase-1
• HSD17
• HSD17B1
• hydroxysteroid (17-beta) dehydrogenase 1 isoform
• hydroxysteroid (17-beta) dehydrogenase 1
• MGC138140
• Placental 17-beta-hydroxysteroid dehydrogenase
• SDR28C1
• short chain dehydrogenase/reductase family 28CE, member 1

Background

The principal human estrogen, 17 beta-estradiol, is a potent stimulator of certain endocrine-dependent forms of breast cancer. Because human estrogenic 17 beta-hydroxysteroid dehydrogenase (HSD17B1) catalyzes the last step in the biosynthesis of 17 beta-estradiol from the less potent estrogen, estrone, it is an attractive target for the design of inhibitors of estrogen production and tumor growth (1). It is concluded that the steroid-binding site of human placental HSD17B1 contains a histidine residue which proximates the upper A-ring region of the steroid as it undergoes the reversible binding step (2). Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies (3). Defects in the conversion of androstenedione to testosterone in the fetal testes by the enzyme HSD17B1 give rise to genetic males with female external genitalia (4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSD17B1