14-3-3 Antibody - BSA Free

Western Blot: 14-3-3 Antibody [NB100-2140] - Analysis of 14-3-3 expression in Hela (A) and Jurkat (B) whole cell lysates.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.2 mg/ml

14-3-3 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide mapping to the amino terminal domain of human 14-3-3.
• Localization: Cytoplasmic
• Specificity: Broadly reactive among the 14-3-3 family, will recognize alpha, beta, zeta (delta) and theta 14-3-3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAQ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:400
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Jurkat Whole Cell Lysate
  HeLa Whole Cell Lysate
  14-3-3 tau/theta Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, with 0.2% gelatin
• Preservative: 0.1% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 14-3-3 Antibody - BSA Free

• 14-3-3 protein tau
• 14-3-3 protein T-cell
• 14-3-3 protein theta
• 1433
• 14-3-3
• 14-3-3,1C5
• HS1
• Protein HS1
• protein tau
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide

Background

14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAQ
• Entrez:
  • 10971(Human)
• Uniprot:
  • P27348()