11 beta-HSD1 Antibody - BSA Free

Western Blot: 11 beta-HSD1 Antibody [NBP3-13393] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with 11 beta-HSD1 antibody (NBP3-13393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: 11 beta-HSD1 Antibody [NBP3-13393] - 11 beta-HSD1 antibody detects 11 beta-HSD1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. 11 beta-HSD1 stained by 11 beta-HSD1 antibody (NBP3-13393) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: 11 beta-HSD1 Antibody [NBP3-13393] - Mouse tissue extract (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 11 beta-HSD1 antibody (NBP3-13393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: 11 beta-HSD1 Antibody [NBP3-13393] - 11 beta-HSD1 antibody detects 11 beta-HSD1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. 11 beta-HSD1 stained by 11 beta-HSD1 antibody (NBP3-13393) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

11 beta-HSD1 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of mouse 11 beta-HSD1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSD11B1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for 11 beta-HSD1 Antibody - BSA Free

• 11 betaHSD1
• 11 beta-HSD1
• 11-beta-hydroxysteroid dehydrogenase 1
• EC 1.1.1
• EC 1.1.1.146
• HDL
• HSD11B1
• HSD11HSD11B
• HSD11LMGC13539
• hydroxysteroid (11-beta) dehydrogenase 1
• member 1
• SDR26C1

Background

HSD11B1 is encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSD11B1