rRNA Antibody (Y10b) - BSA Free

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with rRNA Antibody [Y10b] conjugated to Biotin (NB100-662B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - rRNA antibody was tested in Ntera-2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - Staining of rRNA in vero cells.

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-rRNA (Y10b) conjugated to DyLight 488 [NB100-662G] at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry: rRNA Antibody (Y10b) [NB100-662] - Immunohistochemical staining of mouse neuronal progenitor culture using rRNA antibody (green). Image from verified customer review.

Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA antibody (Y10b) NB100-662 (blue) and an isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis of Alexa Fluor (R) 647 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1%

Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on RAW 246.7 cells with rRNA Antibody (Y10b) NB100-662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on A549 cells with NB100-662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on SH-SY5Y cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

CyTOF-reported: rRNA Antibody (Y10b) [NB100-662] - Human bone marrow after RBC lysis, stained with anti-CD16, fixed, permeabilized with methanol, stained with anti-lactoferrin 1C6 NB120-10109 and anti-rRNA Y10b NB100-662, and gated on myeloid cells per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.

• Reactivity: Hu, Mu, All, Ba
• Applications: Flow, ICC/IF, IHC, IP, CyTOF-ready, ICC/IF
• Clone: Y10b
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

rRNA Antibody (Y10b) - BSA Free Summary

• Immunogen: The whole 5.8S ribosomal RNA.
• Localization: Cytoplasmic. Nuclear.
• Specificity: This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready reported by customer review
  • CyTOF-reported
  • Flow (Intracellular) 2.5 ug/ml
  • Flow Cytometry 2.5 ug/ml
  • Immunocytochemistry 1 - 3 ug/ml
  • Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 34980897)
  • Immunohistochemistry 1:100 - 1:300
  • Immunohistochemistry-Paraffin 1:100 - 1:300
  • Immunoprecipitation 10 ul/lysate
• Application Notes: In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells.

Reactivity Notes

This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for rRNA Antibody (Y10b) - BSA Free

• ribosomal RNA 5.8s

Background

rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of translation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease. Our rRNA (Y10b) antibody recognizes both the large as well as the small subunit of rRNA from all eukaryotic cells and with is applicability in ICC/IF and IP techniques, it provides a valuable molecular tool for rRNA research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for rRNA Antibody (NB100-662)(26)

Publications using NB100-662

• Zhang H, Chen Q, Zhang Q et al. DDX24 Mutation Alters NPM1 Phase Behavior and Disrupts Nucleolar Homeostasis in Vascular Malformations International Journal of Biological Sciences 2023-08-06 [PMID: 37705750]
• Pietraforte I, Butera A, Gaddini L et al. CXCL4-RNA Complexes Circulate in Systemic Sclerosis and Amplify Inflammatory/Pro-Fibrotic Responses by Myeloid Dendritic Cells International Journal of Molecular Sciences 2022-12-30 [PMID: 36614095] (WB, Human)
• Vivanco Gonzalez N, Oliveria JP, Tebaykin D et al. An optimized protocol for phenotyping human granulocytes by mass cytometry STAR protocols [PMID: 35434655]
• Gai X, Xin D, Wu D et al. Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response Cell research 2022-01-04 [PMID: 34980897] (ICC/IF)
• Fischer S, Nasyrov E, Brosien M Et al. Self-extracellular RNA promotes pro-inflammatory response of astrocytes to exogenous and endogenous danger signals Journal of neuroinflammation 2021-11-02 [PMID: 34727934] (IF/IHC)
• Smolka JA, Sanz LA, Hartono SR, ChEdin F Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids The Journal of cell biology 2021-06-07 [PMID: 33830170]
• Fu Y, Zhuang X m6A-binding YTHDF proteins promote stress granule formation Nat. Chem. Biol 2020-05-25 [PMID: 32451507]
  
  Details:
  Citation using the Alexa Fluor 488 format of this antibody.
• Wu D, Dean J EXOSC10 sculpts the transcriptome during the growth-to-maturation transition in mouse oocytes Nucleic Acids Res. 2020-06-04 [PMID: 32313933] (Mouse)
• Cho SS, Yang JH, Seo KH et al. C9orf72 Poly(PR) Dipeptide Repeats Disturb Biomolecular Phase Separation and Disrupt Nucleolar Function Mol. Cell 2019-05-16 [PMID: 30981631]
• Pitchiaya S, Mourao M, Jalihal A et al. Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality Molecular Cell 2019-04-01 [PMID: 30952514] (Human)
• Tsalikis J, Tattoli I, Ling A et al. Intracellular bacterial pathogens trigger the formation of U bodies through metabolic stress induction J. Biol. Chem. 2015-07-01 [PMID: 26134566]
• Fischer S, Gesierich S, Griemert B et al. Extracellular RNA liberates Tumor-Necrosis-Factor-alpha to promote tumor cell trafficking and progression Cancer Res 2013-06-17 [PMID: 23774209] (IHC-P, Human)
• Mastroeni D, Grover A, Delvaux E et al. Epigenetic changes in Alzheimer's disease: decrements in DNA methylation. Neurobiol Aging 2010-12-01 [PMID: 19117641] (Human)
• 9) Thomas, MG, Martinez Tosar, LJ, Loschi, M, Pasquini, JM, Correale, J, Kindler, S, Boccaccio, GL. Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes. Mol Biol Cell;16(1):405-20. 2005-01-01 [PMID: 15525674]
• 7) Sotelo-Silveira, JR, Calliari, A, Cardenas, M, Koenig, E, Sotelo, JR. Myosin Va and kinesin II motor proteins are concentrated in ribosomal domains (periaxoplasmic ribosomal plaques) of myelinated axons. J Neurobiol;60(2):187-96. 2004-08-01 [PMID: 15266650] (ICC/IF, Fish)
• 6) Jablonka, S, Wiese, S, Sendtner, M. Axonal defects in mouse models of motoneuron disease. J Neurobiol;58(2):272-86. 2004-02-05 [PMID: 14704958] (ICC/IF, Mouse)
• 2) Anderson, KD, Merhege, MA, Morin, M, Bolognani, F, Perrone-Bizzozero, NI. Increased expression and localization of the RNA-binding protein HuD and GAP-43 mRNA to cytoplasmic granules in DRG neurons during nerve regeneration. Exp Neurol;183(1):100-8. 2003-09-01 [PMID: 12957493]
• 3) Bleher, R, Martin, R. Ribosomes in the squid giant axon. Neuroscience;107(3):527-34. 2001-01-01 [PMID: 11719007] (ICC/IF, Other, Plant)
• 10) Zheng, J, Kelly, TK, Chang, B, Ryazantsev, S, Rajasekaran, AK, Martin, KC, Twiss, JL. A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons. J Neurosci;21(23):9291-303. 2001-12-01 [PMID: 11717363] (IP, ICC/IF, Rat)
• 1) Aakalu, G, Smith, WB, Nguyen, N, Jiang, C, Schuman, EM. Dynamic visualization of local protein synthesis in hippocampal neurons. Neuron;30(2):489-502. 2001-05-01 [PMID: 11395009] (ICC/IF, Rat)
• 5) Gallouzi, IE, Brennan, CM, Stenberg, MG, Swanson, M, Eversole, A, Maizels, N, Steitz, JA. HuR binding to cytoplasmic mRNA is perturbed by heat shock. Proc Natl Acad Sci U S A. 97(7):3073-8. 2000-03-28 [PMID: 10737787]
• Stone, JS, Rubel, EW. Temporal, spatial, and morphologic features of hair cell regeneration in the avian basilar papilla. J Comp Neurol. 2000-01-01 [PMID: 10660884] (ICC/IF, Chicken)
• 4) Fan, XC, Steitz, JA. Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs. EMBO J;17(12):3448-60. 1998-06-15 [PMID: 9628880] (ICC/IF, Human)
• 12) Abou, ES, Nazar, RN. Role of the 5.8S rRNA in ribosome translocation. Nucleic Acids Res. 25(9):1788-94. 1997-05-01 [PMID: 9108162]
• Garden G, Hartlage-Rubsamen M, Rubel E, Bothwell M Protein masking of a ribosomal RNA epitope is an early event in afferent deprivation-induced neuronal death Mol Cell Neurosci 1995-06-01 [PMID: 7496633] (IHC-P, Chicken)
• 11) Lerner, EA, Lerner, MR, Janeway, CA, Steitz, JA. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. Proc Natl Acad Sci U S A;78(5):2737-41. 1981-05-01 [PMID: 6789322] (IP, Mouse)

Reviews for rRNA Antibody (NB100-662) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Albert Tsai CyTOF Human 03/18/2020

Summary

• Application: CyTOF
• Sample Tested: bone marrow
• Species: Human
• Lot: N/A

reviewed by: Verified Customer IF Mouse 11/16/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse neuronal progenitor culture
• Species: Mouse
• Lot: D-1

FAQs for rRNA Antibody (NB100-662). (Showing 1 - 2 of 2 FAQs).

1. What would the cost be for 100 ug carrier free (no BSA or glycerol) of the Y10b antibody? rRNA Antibody
   • This product does not contain BSA or glycerol and comes in 0.1 ml @ 1mg/ml for $329 in the USA.
2. Hi, could you please explain a little more about rRNA Antibody (Y10b) NB100-662 to me? Is it a protein antibody recognizing 5.8 rRNA structure? Or is it an RNA probe containing specific 5.8S rRNA that can detect rRNA through RNA-FISH method? Thanks!
   • This is an anti-rRNA antibody that was raised against the whole 5.8S ribosomal RNA. It has been tested for Flow, Flow-IC, ICC/IF, IHC-P, and IP. It has not been tested or validated in RNA-FISH method.