rRNA Antibody (Y10b)

Images

 
Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-rRNA (Y10b) conjugated to DyLight ...read more
Flow Cytometry: rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were conjugated ...read more
Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - rRNA antibody was tested in Ntera-2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight ...read more
Immunocytochemistry: rRNA Antibody (Y10b) [NB100-662] - Immunohistochemical staining of mouse neuronal progenitor culture using rRNA antibody (green). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - Staining of rRNA in vero cells.
Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA antibody (Y10b) NB100-662 (blue) and an ...read more
Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis of Alexa Fluor (R) 647 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a ...read more
Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on RAW 246.7 cells with rRNA Antibody (Y10b) NB100-662APC (blue) and a matched isotype control (orange). Cells were fixed ...read more
Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on A549 cells with NB100-662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on SH-SY5Y cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a matched isotype control (orange). Cells were fixed ...read more
CyTOF-ready: rRNA Antibody (Y10b) [NB100-662] - Human bone marrow after RBC lysis, stained with anti-CD16, fixed, permeabilized with methanol, stained with anti-lactoferrin 1C6 NB120-10109 and anti-rRNA Y10b NB100-662, ...read more

Product Details

Summary
Reactivity Hu, Mu, All, BaSpecies Glossary
Applications Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, ICC/IF
Clone
Y10b
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

rRNA Antibody (Y10b) Summary

Immunogen
The whole 5.8S ribosomal RNA.
Localization
Cytoplasmic. Nuclear.
Specificity
This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 3 ug/ml
  • Immunohistochemistry 1:100 - 1:300
  • Immunohistochemistry-Paraffin 1:100 - 1:300
  • Immunoprecipitation 10 ul/lysate
  • CyTOF-ready
  • Flow (Intracellular) 2.5 ug/ml
  • Immunocytochemistry 1 - 3 ug/ml
Application Notes
This rRNA Antibody (Y10b) is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells. This rRNA antibody (Y10b) is validated for CyTOF from a verified customer review.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-662 in the following applications:

Publications
Read Publications using
NB100-662 in the following applications:

  • 7 publications
  • IP
    2 publications

Reactivity Notes

This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for rRNA Antibody (Y10b)

  • ribosomal RNA 5.8s

Background

rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of translation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease. Our rRNA (Y10b) antibody recognizes both the large as well as the small subunit of rRNA from all eukaryotic cells and with is applicability in ICC/IF and IP techniques, it provides a valuable molecular tool for rRNA research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for rRNA Antibody (NB100-662)(17)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Fish, Other, Plant.

We have publications tested in 2 applications: ICC/IF, IP.


Filter By Application
ICC/IF
(7)
IP
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
Rat
(2)
Chicken
(1)
Fish
(1)
Other
(1)
Plant
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-662 Applications Species
Wu D, Dean J EXOSC10 sculpts the transcriptome during the growth-to-maturation transition in mouse oocytes Nucleic Acids Res. Jun 4 2020 [PMID: 32313933] (Mouse) Mouse
Cho SS, Yang JH, Seo KH et al. C9orf72 Poly(PR) Dipeptide Repeats Disturb Biomolecular Phase Separation and Disrupt Nucleolar Function Mol. Cell May 16 2019 [PMID: 30981631]
Pitchiaya S, Mourao M, Jalihal A et al. Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality Molecular Cell Apr 1 2019 [PMID: 30952514] (Human) Human
Tsalikis J, Tattoli I, Ling A et al. Intracellular bacterial pathogens trigger the formation of U bodies through metabolic stress induction J. Biol. Chem. 2015 Jul 01 [PMID: 26134566]
Mastroeni D, Grover A, Delvaux E et al. Epigenetic changes in Alzheimer's disease: decrements in DNA methylation. Neurobiol Aging 2010 Dec [PMID: 19117641] (Human) Human
9) Thomas, MG, Martinez Tosar, LJ, Loschi, M, Pasquini, JM, Correale, J, Kindler, S, Boccaccio, GL. Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes. Mol Biol Cell;16(1):405-20. 2005 Jan. [PMID: 15525674]
7) Sotelo-Silveira, JR, Calliari, A, Cardenas, M, Koenig, E, Sotelo, JR. Myosin Va and kinesin II motor proteins are concentrated in ribosomal domains (periaxoplasmic ribosomal plaques) of myelinated axons. J Neurobiol;60(2):187-96. 2004 Aug. [PMID: 15266650] (ICC/IF, Fish) ICC/IF Fish
6) Jablonka, S, Wiese, S, Sendtner, M. Axonal defects in mouse models of motoneuron disease. J Neurobiol;58(2):272-86. 2004 Feb 5. [PMID: 14704958] (ICC/IF, Mouse) ICC/IF Mouse
2) Anderson, KD, Merhege, MA, Morin, M, Bolognani, F, Perrone-Bizzozero, NI. Increased expression and localization of the RNA-binding protein HuD and GAP-43 mRNA to cytoplasmic granules in DRG neurons during nerve regeneration. Exp Neurol;183(1):100-8. 2003 Sep. [PMID: 12957493]
3) Bleher, R, Martin, R. Ribosomes in the squid giant axon. Neuroscience;107(3):527-34. 2001 [PMID: 11719007] (ICC/IF, Plant, Other) ICC/IF Plant, Other
Show All 17 Publications.

Reviews for rRNA Antibody (NB100-662) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-662:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 rRNA NB100-662
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reviewed by:
Albert Tsai
Human 03/18/2020
View

Summary

Sample TestedBone marrow
SpeciesHuman
LotN/A
Immunofluorescence rRNA NB100-662
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reviewed by:
Anonymous
IF Mouse 11/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse neuronal progenitor culture
SpeciesMouse
LotD-1

Blocking

Blocking DetailsNGS

Primary Anitbody

Dilution Ratio1:100

Secondary Antibody

Secondary DescriptionMouse 488

Details

Detection NotesWide Field Microscope
Fixation DetailsPFA fixation
Wash DescriptionPBS

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for rRNA Antibody (NB100-662). (Showing 1 - 2 of 2 FAQs).

  1. What would the cost be for 100 ug carrier free (no BSA or glycerol) of the Y10b antibody? rRNA Antibody
    • This product does not contain BSA or glycerol and comes in 0.1 ml @ 1mg/ml for $329 in the USA.
  2. Hi, could you please explain a little more about rRNA Antibody (Y10b) NB100-662 to me? Is it a protein antibody recognizing 5.8 rRNA structure? Or is it an RNA probe containing specific 5.8S rRNA that can detect rRNA through RNA-FISH method? Thanks!
    • This is an anti-rRNA antibody that was raisedĀ againstĀ the whole 5.8S ribosomal RNA. It has been tested for Flow, Flow-IC, ICC/IF, IHC-P, and IP. It has not been tested or validated in RNA-FISH method.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-662AF350
Alexa Fluor 405 NB100-662AF405
Alexa Fluor 488 NB100-662AF488
Alexa Fluor 532 NB100-662AF532
Alexa Fluor 594 NB100-662AF594
Alexa Fluor 647 NB100-662AF647
Alexa Fluor 700 NB100-662AF700
Alexa Fluor 750 NB100-662AF750
Allophycocyanin NB100-662APC
Allophycocyanin/Cy7 NB100-662APCCY7
Biotin NB100-662B
DyLight 350 NB100-662UV
DyLight 405 NB100-662V
DyLight 488 NB100-662G
DyLight 550 NB100-662R
DyLight 594 NB100-662DL594
DyLight 650 NB100-662C
DyLight 680 NB100-662FR
DyLight 755 NB100-662IR
FITC NB100-662F
HRP NB100-662H
Janelia Fluor 549 NB100-662JF549
Janelia Fluor 646 NB100-662JF646
PE NB100-662PE
PE/Atto594 NB100-662PEATT594
PE/Cy5.5 NB100-662PECY55
PE/Cy7 NB100-662PECY7

Additional rRNA Products

Array NB100-662

Research Areas for rRNA Antibody (NB100-662)

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Recent Reviews

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1
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Albert Tsai
03/18/2020
Application:
Species: Human

Anonymous
11/16/2015
Application: IF
Species: Mouse