rRNA Antibody (Y10b)


Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - Staining of rRNA in vero cells.
Immunohistochemistry: rRNA Antibody (Y10b) [NB100-662] - Immunohistochemical staining of mouse neuronal progenitor culture using rRNA antibody (green). Image from verified customer review.
Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA antibody (Y10b) NB100-662 (blue) and an ...read more
Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - rRNA antibody was tested in Ntera-2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more

Product Details

Reactivity Hu, Mu, AllSpecies Glossary
Applications Flow-IC, ICC/IF, IHC, IHC-P, IP
1 mg/ml

rRNA Antibody (Y10b) Summary

The whole 5.8S ribosomal RNA.
Cytoplasmic. Nuclear.
This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
IgG3 Kappa
Protein A purified
Innovator's Reward
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
1M Tris, 1.5M NaCl, 100mM Glycine
0.05% Sodium Azide
1 mg/ml
Protein A purified


Application Notes
This rRNA Antibody (Y10b) is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells.
Reviewed Applications
Read 1 Review rated 5
NB100-662 in the following application:

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NB100-662 in the following applications:

Reactivity Notes

This antibody reacts with all eukaryotes.

Alternate Names for rRNA Antibody (Y10b)

  • ribosomal RNA 5.8s


rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of translation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease. Our rRNA (Y10b) antibody recognizes both the large as well as the small subunit of rRNA from all eukaryotic cells and with is applicability in ICC/IF and IP techniques, it provides a valuable molecular tool for rRNA research.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for rRNA Antibody (NB100-662)(16)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Fish, Other, Plant.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, IP.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-662 Applications Species
Tsalikis J, Tattoli I, Ling A et al. Intracellular bacterial pathogens trigger the formation of U bodies through metabolic stress induction J. Biol. Chem. 2015 Jul 01 [PMID:26134566]
Fischer S, Gesierich S, Griemert B et al. Extracellular RNA liberates Tumor-Necrosis-Factor-alpha to promote tumor cell trafficking and progression. Cancer Res 2013 Jun 17 [PMID:23774209] (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human
Mastroeni D, Grover A, Delvaux E et al. Epigenetic changes in Alzheimer's disease: decrements in DNA methylation. Neurobiol Aging 2010 Dec [PMID:19117641] (Human) Human
9) Thomas, MG, Martinez Tosar, LJ, Loschi, M, Pasquini, JM, Correale, J, Kindler, S, Boccaccio, GL. Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes. Mol Biol Cell;16(1):405-20. 2005 Jan. [PMID:15525674]
7) Sotelo-Silveira, JR, Calliari, A, Cardenas, M, Koenig, E, Sotelo, JR. Myosin Va and kinesin II motor proteins are concentrated in ribosomal domains (periaxoplasmic ribosomal plaques) of myelinated axons. J Neurobiol;60(2):187-96. 2004 Aug. [PMID:15266650] (ICC/IF, Fish) ICC/IF Fish
6) Jablonka, S, Wiese, S, Sendtner, M. Axonal defects in mouse models of motoneuron disease. J Neurobiol;58(2):272-86. 2004 Feb 5. [PMID:14704958] (ICC/IF, Mouse) ICC/IF Mouse
2) Anderson, KD, Merhege, MA, Morin, M, Bolognani, F, Perrone-Bizzozero, NI. Increased expression and localization of the RNA-binding protein HuD and GAP-43 mRNA to cytoplasmic granules in DRG neurons during nerve regeneration. Exp Neurol;183(1):100-8. 2003 Sep. [PMID:12957493]
3) Bleher, R, Martin, R. Ribosomes in the squid giant axon. Neuroscience;107(3):527-34. 2001 [PMID:11719007] (ICC/IF, Plant, Other) ICC/IF Plant, Other
10) Zheng, J, Kelly, TK, Chang, B, Ryazantsev, S, Rajasekaran, AK, Martin, KC, Twiss, JL. A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons. J Neurosci;21(23):9291-303. 2001 Dec 1. [PMID:11717363] (ICC/IF, IP, Rat) ICC/IF, IP Rat
1) Aakalu, G, Smith, WB, Nguyen, N, Jiang, C, Schuman, EM. Dynamic visualization of local protein synthesis in hippocampal neurons. Neuron;30(2):489-502. 2001 May. [PMID:11395009] (ICC/IF, Rat) ICC/IF Rat
Show All 16 Publications.

Reviews for rRNA Antibody (NB100-662) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-662:
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Images Ratings Applications Species Date Details
 rRNA NB100-662
reviewed by:
Mouse 11/16/2015


Sample TestedMouse neuronal progenitor culture


Blocking DetailsNGS

Primary Anitbody

Dilution Ratio1:100

Secondary Antibody

Secondary DescriptionMouse 488


Detection NotesWide Field Microscope
Fixation DetailsPFA fixation
Wash DescriptionPBS

Product General Protocols

View specific protocols for rRNA Antibody (NB100-662): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for rRNA Antibody (NB100-662) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-662AF405
Alexa Fluor (R) 488 Labeled NB100-662AF488
Alexa Fluor (R) 647 Labeled NB100-662AF647
Alexa Fluor (R) 700 Labeled NB100-662AF700
Allophycocyanin Labeled NB100-662APC
Biotin Labeled NB100-662B
DyLight 350 Labeled NB100-662UV
DyLight 405 Labeled NB100-662V
DyLight 405LS Labeled NB100-662V3
DyLight 488 Labeled NB100-662G
DyLight 550 Labeled NB100-662R
DyLight 650 Labeled NB100-662C
DyLight 680 Labeled NB100-662FR
DyLight 755 Labeled NB100-662IR
FITC Labeled NB100-662F
HRP Labeled NB100-662H
PE Labeled NB100-662PE
PerCP Labeled NB100-662PCP

Additional rRNA Antibody (Y10b) Products

rRNA NB100-662

Research Areas for rRNA Antibody (NB100-662)

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Blogs on rRNA

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