Delta Np73 Antibody (38C674.2) - Azide and BSA Free

Western Blot: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - Delta Np73 Antibody (38C674.2) analysis for Np73 using NBP2-24873 at 1 ug/ml in A) a cell line transfected with Np73 cDNA, B) HeLa, and C) NIH 3T3 cell lysate. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Delta Np73 (38C674.2) NBP2-24873 at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - Delta Np73 Antibody (38C674.2) Formalin-fixed, paraffin-embedded lung squamous cell carcinoma probed with p73 antibody at 5 ug/ml. Image from the standard format of this antibody.

Immunohistochemistry: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - Delta Np73 Antibody (38C674.2) analysis of Delta Np73 in embryonic day 15 (A) and day 17 (B) E15 rat embryonic kidney at 1:2000. Delta Np73 is expressed in ureteric bud (UB) branches, and metanephrogenic mesenchyme (MM), as well as in cells invading the g

Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) - Azide Free [NBP2-33262] - Delta Np73 Antibody (38C674.2) Staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 38C674.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

Delta Np73 Antibody (38C674.2) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Delta Np73 Antibody (38C674.2) - Azide and BSA Free (NBP2-24873) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a peptide corresponding to a portion of amino acid residues 1-50 of human dNp73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP73
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-3 ug/ml
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Delta Np73 Antibody (38C674.2) - Azide and BSA Free

• p53-related protein
• p73
• tumor protein p73

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP73