rRNA Antibody (Y10b) [DyLight 405]

• Reactivity: Hu, Mu, All, Ba
• Applications: Flow, ICC/IF, IHC, IP, CyTOF-ready, ICC/IF
• Clone: Y10b
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 405

rRNA Antibody (Y10b) [DyLight 405] Summary

• Immunogen: The whole 5.8S ribosomal RNA.
• Localization: Cytoplasmic. Nuclear.
• Specificity: This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • CyTOF-reported
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for rRNA Antibody (Y10b) [DyLight 405]

• ribosomal RNA 5.8s

Background

rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of transtlation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease. Our rRNA (Y10b) antibody recognizes both the large as well as the small subunit of rRNA from all eukaryotic cells and with is applicability in ICC/IF and IP techniques, it provides a valuable molecular tool for rRNA research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.