TBK1 Antibody (108A429) - Azide and BSA Free

Western Blot: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TBK1 at 20 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.

Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. Image using the standard format of this product.

Western Blot: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x. Image using the standard format of this product.

Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE))

Simple Western: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Western Blot: NAK Antibody (108A429) [NB100-56705] - Simple Western lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm
• Applications: WB, Simple Western, ICC/IF, IHC, KO
• Clone: 108A429
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

TBK1 Antibody (108A429) - Azide and BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse TBK1 Antibody (108A429) - Azide and BSA Free (NB100-56705) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TBK1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockout Validated
  • Simple Western
  • Western Blot 1-2 ug/ml
• Application Notes: In Western Blot this antibody detects a band at 80 kDa.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS - BSA free
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TBK1 Antibody (108A429) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• FLJ11330
• FTDALS4
• NAK serine/threonine-protein kinase TBK 1
• NAK
• NAKserine/threonine-protein kinase TBK1
• NF-kappa-B-activating kinase
• NFKB-Activating Kinase
• T2K
• TANK-binding kinase 1NF-kB-activating kinase
• TBK 1
• TBK1

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TBK1 Antibody (NBP2-33243). (Showing 1 - 6 of 6 FAQ).

1. What is the exact sequence this TBK1 antibody recognizes?
   • The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
2. I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
   • While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
3. We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
   • Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
4. Is there a smaller size of your TBK1 antibody?
   • This TBK1 antibody is offered in a smaller 0.025 mg size.
5. Are there any publications where this TBK1 antibody was used in western blot for mouse?
   • This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
6. Cant this TBK1 antibody be conjugated to PE for FLOW?
   • Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

Bioinformatics

• Gene Symbol: TBK1
• Entrez:
  • 29110(Human)
  • 56480(Mouse)
  • 299827(Rat)
• Uniprot:
  • Q9UHD2()