rRNA Antibody (Y10b) [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, AllSpecies Glossary
Applications ICC/IF, IP
Clone
Y10b
Clonality
Monoclonal
Host
Mouse

rRNA Antibody (Y10b) [DyLight 550] Summary

Immunogen
The whole 5.8S ribosomal RNA.
Localization
Cytoplasmic. Nuclear.
Specificity
This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 550
Format
DyLight 550 (A=562, E=576)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:2500
  • Immunoprecipitation 10 ul/lysate
Application Notes
This rRNA Antibody (Y10b) is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells.

Reactivity Notes

This antibody reacts with all eukaryotes.

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for rRNA Antibody (Y10b) [DyLight 550]

  • ribosomal RNA 5.8s

Background

The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro. This antibody is useful for recognizing RNA epitopes on both the large and small subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for rRNA Antibody (NB100-662R) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-662APC
Biotin Labeled NB100-662B
DyLight 350 Labeled NB100-662UV
DyLight 405 Labeled NB100-662V
DyLight 405LS Labeled NB100-662V3
DyLight 488 Labeled NB100-662G
DyLight 650 Labeled NB100-662C
DyLight 680 Labeled NB100-662FR
DyLight 755 Labeled NB100-662IR
FITC Labeled NB100-662F
HRP Labeled NB100-662H
PE Labeled NB100-662PE
PerCP Labeled NB100-662PCP

Additional rRNA Antibody (Y10b) [DyLight 550] Products

rRNA NB100-662R

Blogs on rRNA

There are no specific blogs for rRNA, but you can read our latest blog posts.

Contact Information

Product PDFs

Customer Resources

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