rRNA Antibody (Y10b) [PerCP]

• Reactivity: Hu, Mu, All, Ba
• Applications: Flow, ICC/IF, IHC, IP, CyTOF-ready, ICC/IF
• Clone: Y10b
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PerCP

rRNA Antibody (Y10b) [PerCP] Summary

• Immunogen: The whole 5.8S ribosomal RNA.
• Localization: Cytoplasmic. Nuclear.
• Specificity: This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • CyTOF-reported
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: This rRNA Antibody (Y10b) is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells.

Reactivity Notes

This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for rRNA Antibody (Y10b) [PerCP]

• ribosomal RNA 5.8s

Background

The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro. This antibody is useful for recognizing RNA epitopes on both the large and small subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.