rRNA Antibody (Y10b) [Janelia Fluor® 525]

• Reactivity: Hu, Mu, All, Ba
• Applications: Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: Y10b
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

rRNA Antibody (Y10b) [Janelia Fluor® 525] Summary

• Immunogen: The whole 5.8S ribosomal RNA.
• Specificity: This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • CyTOF-reported
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for rRNA Antibody (Y10b) [Janelia Fluor® 525]

• ribosomal RNA 5.8s

Background

The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro. This antibody is useful for recognizing RNA epitopes on both the large and small subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.