Osteoactivin/GPNMB Antibody [Unconjugated]

Images

 
Western blot shows lysates of U‑118‑MG human glioblastoma/astrocytoma cell line and T98G human glioblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Osteoactivin/GPNMB Antigen ...read more
Osteoactivin/GPNMB was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) at 3 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Osteoactivin/GPNMB Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Osteoactivin isoform b
Lys23-Asn486
Accession # NP_002501
Specificity
Detects human Osteoactivin in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant mouse Osteoactivin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GPNMB
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human Osteoactivin/GPNMB Antibody (Catalog # AF2550)
ELISA Detection: Human Osteoactivin/GPNMB Biotinylated Antibody (Catalog # BAF2550)
Standard: Recombinant Human Osteoactivin/GPNMB Fc Chimera (Catalog # 2550-AC)
Publications
Read Publications using
AF2550 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteoactivin/GPNMB Antibody [Unconjugated]

  • DC-HIL
  • glycoprotein (transmembrane) nmb
  • glycoprotein NMB
  • GPNMB
  • HGFIN
  • HGFINglycoprotein nmb-like protein
  • NMBosteoactivin
  • Osteoactivin
  • Transmembrane glycoprotein HGFIN
  • transmembrane glycoprotein NMB

Background

Osteoactivin (also GPNMB and DC-HIL) is a variably glycosylated 75 - 125 kDa member of the NMB/pMEL-17 family of molecules. It is found in multiple subcellular sites, but is most often associated with the endosomal/lysosomal compartment (1-3). Human Osteoactivin is a 560 amino acid (aa) type I transmembrane protein. Its precursor contains a 21 aa signal sequence, a 465 aa luminal/extracellular domain, a 21 aa transmembrane segment and a 53 aa cytoplasmic tail (4, 5). The luminal region contains an N-terminal heparin-binding motif (aa 23-26), multiple glycosylation sites, an RGD motif (aa 64-66) and an 88 aa PKD domain (aa 240-327). The intracellular tail has an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif (4, 5). The extracellular/luminal region shares 74% and 77% aa identity with the equivalent regions in mouse and canine, respectively. Multiple isoforms would appear to exist. There is one alternate splice form known that shows a 12 aa insert between aa 339-340 (6). An addtional 206 aa isoform shows a mutation at position # 181 that results in a 26 aa substitution for the C-terminal 380 amino acids (7, 8). This has the potential to be soluble and may represent a counterpart to a secreted isoform of rat Osteoactivin (9). Cells known to express Osteoactivin include macrophages/Kupffer cells, fibroblasts, osteoblasts, myeloid dendritic cells, retinal pigment epithelial cells and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (3-5, 10-12). In mice, Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of endothelial cells and may also interact with integrins (13). It also appears to act as an inflammatory suppressor gene, as its expression downregulates the macrophage inflammatory response by inhibiting IL-6 and IL-12 p40 production (3).

  1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
  2. Safadi, F.F. et al. (2002) J. Cell. Biochem. 84:12.
  3. Ripoll, V.M. et al. (2007) J. Immunol. 178:6557. 
  4. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205. 
  5. Weterman, M.A.J. et al. (1995) Int. J. Cancer 60:73. 
  6. Kuan, C-T. et al. (2006) Clin. Cancer Res. 12:1970. 
  7. Lennerz, V. et al. (2005) Proc. Natl. Acad. Sci. USA 102:16013.
  8. Genbank Accession # AAH11595.
  9. Abdelmagid, S.M. et al. (2007) J. Cell. Physiol. 210:26.
  10. Haralanova-Ilieva, B. et al. (2005) J. Hepatol. 42:565.
  11. Ahn, J.H. et al. (2002) Blood 100:1742.
  12. Anderson, M.G. et al. (2002) Nat. Genet. 30:81.
  13. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Osteoactivin/GPNMB Antibody (AF2550)(16)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Xenograft.

We have publications tested in 6 applications: FACS, Flow Cytometry, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
FACS
(1)
Flow Cytometry
(1)
IHC
(3)
IHC-Fr
(1)
IHC-P
(3)
Western Blot
(7)
All Applications
Filter By Species
Human
(13)
Mouse
(1)
N/A
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF2550 Applications Species
M Lang, CD Vocke, CJ Ricketts, AR Metwalli, MW Ball, LS Schmidt, WM Linehan Clinical and Molecular Characterization of Microphthalmia-Associated Transcription Factor (MITF)-Related Renal Cell Carcinoma Urology, 2020;0(0):. 2020 [PMID: 33242557] (IHC, Human) IHC Human
M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations Acta Neuropathol Commun, 2020;8(1):163. 2020 [PMID: 33028409] (Western Blot, Human) Western Blot Human
KB Biswas, A Takahashi, Y Mizutani, S Takayama, A Ishitsuka, L Yang, F Yang, A Iddamalgod, I Katayama, S Inoue GPNMB is expressed in human epidermal keratinocytes but disappears in the vitiligo lesional skin Sci Rep, 2020;10(1):4930. 2020 [PMID: 32188902] (Western Blot, Human) Western Blot Human
R Xie, Y Okita, Y Ichikawa, MA Fikry, KT Huynh Dam, STP Tran, M Kato Role of the kringle-like domain in glycoprotein NMB for its tumorigenic potential Cancer Sci., 2019;110(7):2237-2246. 2019 [PMID: 31127873] (Flow Cytometry, Mouse) Flow Cytometry Mouse
M Baba, M Furuya, T Motoshima, M Lang, S Funasaki, W Ma, HW Sun, H Hasumi, Y Huang, I Kato, T Kadomatsu, Y Satou, N Morris, BO Karim, L Ileva, JD Kalen, LA Wilan Kris, Y Hasumi, A Sugiyama, R Kurahashi, K Nishimoto, M Oyama, Y Nagashima, N Kuroda, K Araki, M Eto, M Yao, T Kamba, T Suda, Y Oike, LS Schmidt, WM Linehan TFE3 Xp11.2 translocation renal cell carcinoma mouse model reveals novel therapeutic targets and identifies GPNMB as a diagnostic marker for human disease Mol. Cancer Res., 2019;0(0):. 2019 [PMID: 31043488] (IHC, Human) IHC Human
H Ashktorab, H Rahi, M Nouraie, B Shokrani, E Lee, T Haydari, AO Laiyemo, P Siegel, H Brim GPNMB methylation: a new marker of potentially carcinogenic colon lesions BMC Cancer, 2018;18(1):1068. 2018 [PMID: 30400781] (IHC, Human) IHC Human
C Chen, Y Okita, Y Watanabe, F Abe, MA Fikry, Y Ichikawa, H Suzuki, A Shibuya, M Kato Glycoprotein nmb is exposed on the surface of dormant breast cancer cells and induces stem cell-like properties Cancer Res., 2018;0(0):. 2018 [PMID: 30224376] (FACS, Human) FACS Human
X Ye, BT Luke, BR Wei, JA Kaczmarczy, J Loncarek, JE Dwyer, DJ Johann, RG Saul, DV Nissley, F McCormick, GR Whiteley, J Blonder Direct molecular dissection of tumor parenchyma from tumor stroma in tumor xenograft using mass spectrometry-based glycoproteomics Oncotarget, 2018;9(41):26431-26452. 2018 [PMID: 29899869] (IHC-P, Xenograft) IHC-P Xenograft
JY Tajima, M Futamura, S Gaowa, R Mori, T Tanahashi, Y Tanaka, N Matsuhashi, T Takahashi, K Yamaguchi, T Miyazaki, K Yoshida Clinical Significance of Glycoprotein Non-metastatic B and Its Association with EGFR/HER2 in Gastrointestinal Cancer J Cancer, 2018;9(2):358-366. 2018 [PMID: 29344282] (Western Blot, Human) Western Blot Human
J Ma, AR Baker, A Calabro, KA Derwin Exploratory study on the effect of osteoactivin on muscle regeneration in a rat volumetric muscle loss model PLoS ONE, 2017;12(4):e0175853. 2017 [PMID: 28426701] (IHC-Fr, N/A) IHC-Fr N/A
Show All 16 Publications.

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Biotin BAF2550

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Bioinformatics

Gene Symbol GPNMB
Uniprot