Monoclonal Antibodies

By Rosa Moreno, PhD.

Most commercially available antibodies produced with traditional immunization technology, using peptides or other immunogens isolated and purified from cell lysates.

Typically, mice are immunized with the antigen in groups, varying the mouse strain and antigen dosage. Pre-immune and immune sera are evaluated and mice showing the best immune response are selected for somatic cell fusion. This is the fusion of antibody-producing lymphocytes with proprietary myeloma cells from a non-antibody producing source, creating a hybridoma.

What Are Rabbit Monoclonal Antibodies?