Integrin beta 1/CD29 Antibody Summary
Immunogen |
Purified Human beta1 integrin. |
Marker |
Stem Cell Marker |
Specificity |
Recognizes Human Integrin beta1 as confirmed with beta1 transfectants and by reciprocal preclearing of beta1 reactivity with anti-beta1. Species cross-reactivity: Monkey, cynomolgus monkey, rhesus monkey and weakly with porcine CD29. Does not cross-react with rat CD29. |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
ITGB1 |
Purity |
Ammonium sulfate precipitation |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 0.1 - 1 ug/ml
- Flow Cytometry 1:10 - 1:1000
- Immunohistochemistry 1:10 - 1:500
- Immunohistochemistry-Frozen 1:10 - 1:500
- Immunohistochemistry-Paraffin 10 ug/ml
- Immunoprecipitation 1 - 5 ug/ml
- Western Blot 1:100 - 1:2000
|
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.4) |
Preservative |
0.09% Sodium Azide |
Purity |
Ammonium sulfate precipitation |
Alternate Names for Integrin beta 1/CD29 Antibody
Background
CD29 is a 130 kD protein, also known as integrin beta-1, VLA-beta chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMbeta1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for Integrin beta 1/CD29 Antibody (NBP1-50305) (0)
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Product General Protocols
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Video Protocols
FAQs for Integrin beta 1/CD29 Antibody (NBP1-50305). (Showing 1 - 1 of 1 FAQ).
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I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
- I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.