| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-32732-0.1mg | |||
| IHC-Prediluted | NBP2-44450 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47423SS | |||
| Azide and BSA Free | NBP2-80595 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74682SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82574-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78159SS | |||
| Azide and BSA Free | NBP2-80833 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1642SS | |||
| Azide and BSA Free | NBP2-80639 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-210SS | |||
| BSA Free | NB500-210-0.1ml | |||
| Azide and BSA Free | NBP2-80614 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-270SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47386SS | |||
| Azide and BSA Free | NBP2-80650 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05197SS | |||
![Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.](http://images.novusbio.com/fullsize/MUC5AC-Antibody-45M1-Immunohistochemistry-Paraffin-NBP2-15196-img0006.jpg)
![Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded Rat stomach stained with MUC5AC Monoclonal Antibody (45M1).](http://images.novusbio.com/fullsize/MUC5AC-Antibody-45M1-Immunohistochemistry-Paraffin-NBP2-15196-img0009.jpg)
![Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).](http://images.novusbio.com/fullsize/MUC5AC-Antibody-45M1-Immunohistochemistry-Paraffin-NBP2-15196-img0011.jpg)
![Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15196_mouse-monoclonal-muc5ac-antibody-45m1-31020241611350.jpg)
![Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15196_mouse-monoclonal-muc5ac-antibody-45m1-310202416114790.jpg)
![Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15196_mouse-monoclonal-muc5ac-antibody-45m1-310202416121974.jpg)
![Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis using anti-Beta Actin mAb against human, mouse, hamster, rat, and primate cell lystate(s): (1) NIH/3T3, (2) Jurkat, (3) HeLa, (4) CHO, (5) PC12, (6) HEK293, (7) COS, (8) A549, and (9) MCF-7. A specific band can be observed at a molecular weight of approximately 43 kDa in all lanes.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Western-Blot-NBP1-47423-img0006.jpg "Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis using anti-Beta Actin mAb against human, mouse, hamster, rat, and primate cell lystate(s): (1) NIH/3T3, (2) Jurkat, (3) HeLa, (4) CHO, (5) PC12, (6) HEK293, (7) COS, (8) A549, and (9) MCF-7. A specific band can be observed at a molecular weight of approximately 43 kDa in all lanes.")
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (8H10D10) [NBP1-47423] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NBP1-47423) at 1:5400 dilution. Cells were stained using NorthernLights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Immunocytochemistry-Immunofluorescence-NBP1-47423-img0011.jpg)
![Simple Western: beta-Actin Antibody (8H10D10) [NBP1-47423] - Image shows a specific band for Beta Actin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Simple-Western-NBP1-47423-img0009.jpg)
![Flow Cytometry: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (Orange) and Isotype control Antibody (Blue).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Flow-Cytometry-NBP1-47423-img0010.jpg)
![Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - TTF-1 alters the phosphorylation of GSK3alpha/beta. (A) Panels of immunoblot images examining total GSK3alpha/beta and phosphorylation at specific Ser or Tyr residues in A549 transfectant cells. Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-44549-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Western-Blot-NBP1-47423-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (8H10D10) [NBP1-47423] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with beta-Actin Antibody [8H10D10] conjugated to Biotin (NBP1-47423B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Immunocytochemistry-Immunofluorescence-NBP1-47423-img0015.jpg)
![Flow Cytometry: beta-Actin Antibody (8H10D10) - BSA Free [NBP1-47423] - An intracellular stain was performed on NIH3T3 cells with beta-Actin Antibody (8H10D10) NBP1-47423 (blue) and a matched isotype control MAB004 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-BSA-Free-Flow-Cytometry-NBP1-47423-img0017.jpg)
![Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Western blot of pancreatic cell lines (CAPAN2, PATU8988T, DANG, and PANC1005). Primary antibody: beta-Actin (8H10D10), NBP1-47423, 1:1000 dilution. Secondary antibody: HRP conjugated Mouse IgG (H+L), NB7539. A specific band can be observed at a molecular weight of approximately 42 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Western-Blot-NBP1-47423-img0007.jpg)
![Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis of Beta Actin expression in 1) HeLa 2) HepG2 and 3) Cos7 whole cell lysates.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Western-Blot-NBP1-47423-img0008.jpg)
![Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line were probed with 1:2500 mouse anti-beta-Actin monoclonal (NBP1-47423) followed by 1:2000 dilution of donkey anti-mouse IgG-HRP secondary antibody (HAF018).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Western-Blot-NBP1-47423-img0012.jpg)
![Western Blot: beta-Actin Antibody (8H10D10) [NBP1-47423] - Three different human cancer cell lines (SW780, BT-20, and CLS439) were probed with the antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Western-Blot-NBP1-47423-img0013.jpg)
![Flow Cytometry: beta-Actin Antibody (8H10D10) [NBP1-47423] - Analysis of MCF-7 cells using anti-Beta Actin mAb (right) and negative control (left).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-Flow-Cytometry-NBP1-47423-img0004.jpg)
![Flow Cytometry: beta-Actin Antibody (8H10D10) - BSA Free [NBP1-47423] - An intracellular stain was performed on U-251 MG cells with beta-Actin Antibody (8H10D10) NBP1-47423 (blue) and a matched isotype control MAB004 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-8H10D10-BSA-Free-Flow-Cytometry-NBP1-47423-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74682-img0005.jpg "Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74682-img0006.jpg)
![Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts. Cortex (CX), Outer Medulla (OM), Inner Medulla Base (IMB), Inner Medulla Papilla (IMP).](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Western-Blot-NB110-74682-img0001.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NB110-74682] - Analysis of FFPE human kidney using 1:100 conc. of Aquaporin-2 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Lumenal staining of Aquaporin-2 in scattered tubules was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunohistochemistry-Paraffin-NB110-74682-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - Staining of mpkCCD cell line](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74682-img0003.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Analysis in human colon and liver tissues using NBP1-82574 antibody. Corresponding SLC9A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Analysis in human colon and liver tissues using NBP1-82574 antibody. Corresponding SLC9A3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human colon, kidney, liver and small intestine using Anti-SLC9A3 antibody NBP1-82574 (A) shows similar protein distribution across tissues to independent antibody NBP1-82575 (B).](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0025.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human liver shows no membranous positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0028.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human colon shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0024.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human gallbladder shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0026.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human kidney shows weak positivity in apical membrane in cells in tubules.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0027.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0029.jpg)
![Western Blot: LDL R Antibody (C7) [NBP1-78159] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-LDL receptor in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LDL-R-Antibody-C7-Western-Blot-NBP1-78159-img0008.jpg "Western Blot: LDL R Antibody (C7) [NBP1-78159] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-LDL receptor in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: LDLR Antibody (C7) [NBP1-78159] - Immunocytochemistry of LA-6 and intact MPEFs (used as positive control) using anti-LDLR antibody. The parental MPEFs reactive to the secondary antibody (fluorescein-labeled anti-mouse IgG) alone are designated as negative control. All cells were counterstained with DAPI upon reaction with the secondary antibody. Bar = 30 um. Image collected and cropped by CiteAb from the following publication (www.mdpi.com/1422-0067/18/12/2610) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LDLR-Antibody-C7-Immunocytochemistry-Immunofluorescence-NBP1-78159-img0010.jpg)
![Immunohistochemistry: LDL R Antibody (C7) [NBP1-78159] - Analysis of an FFPE tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining.](http://images.novusbio.com/fullsize/LDL-R-Antibody-C7-Immunohistochemistry-NBP1-78159-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Antibody was tested in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/LDL-R-Antibody-C7-Immunocytochemistry-Immunofluorescence-NBP1-78159-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2.](http://images.novusbio.com/fullsize/LDL-R-Antibody-C7-Immunocytochemistry-Immunofluorescence-NBP1-78159-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] (NB100-1642) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0014.jpg "Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] (NB100-1642) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS-1 Cells with CD31/PECAM-1 Antibody (MEC 7.46) NB100-1642(blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Flow-Cell-Surface-NB100-1642-img0005.jpg)
![Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Immunohistochemistry for vessel staining (CD31) and beta-amyloid comparing 13- and 24-month old arcAbeta mice. Overviews (scale bars = 200 um) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnagi.2015.00241/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] conjugated to Alexa Fluor 488 (NB100-1642AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with at 5.0 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] conjugated to DyLight 550 (NB100-1642R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0016.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature. Image using the Alexa Fluor 647 format of this antibody.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0012.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC 7.46] Antibody NB100-1642AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Flow-Cytometry-NB100-1642-img0015.jpg)
![Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Immunohistochemistry for vessel staining (CD31/PECAM-1) and beta-amyloid comparing 13- and 24-month old non-transgenic littermates. Overviews (scale bars = 200 um) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnagi.2015.00241/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0010.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM-1 Antibody (MEC 7.46) was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Flow-Cytometry-NB100-1642-img0006.jpg)
![Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - ALM induces HIF-1alpha-dependent apoptosis in PC3 cells. PC3 cells were treated with ALM for 24 h and 48 h at 0.1 uM under normoxia and hypoxia, followed by Western blot for caspase-3. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/5/439), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Western-Blot-NB500-210-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - ALM induces HIF-1alpha-dependent apoptosis in PC3 cells. PC3 cells were treated with ALM for 24 h and 48 h at 0.1 uM under normoxia and hypoxia, followed by Western blot for caspase-3. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/5/439), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Simple Western: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Lane view shows a specific band for Caspase 3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Simple-Western-NB500-210-img0005.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Analysis of a FFPE human spleen section using 1:200 dilution of . The staining was developed using HRP conjugated anti-mouse secondary antibody and DAB reagent. This Caspase 3 antibody generated a specific staining in the cytoplasm of various spleenocytes.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Immunohistochemistry-Paraffin-NB500-210-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder 1:300 dilution of mouse monoclonal Caspase-3 Antibody (CPP32 4-1-18) (NB500-210, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Immunohistochemistry-Paraffin-NB500-210-img0006.jpg)
![Immunohistochemistry-Frozen: Caspase-3 Antibody (CPP32 4-1-18) - BSA Free [NB500-210] - Analysis of murine spleen with Caspase-3 (green), CD3 (red) and DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-BSA-Free-Immunohistochemistry-Frozen-NB500-210-img0009.jpg)
![Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Detection of Caspase (19 and 35 kDa) from HEK293 cell extract using (NB500-210). Lanes 1 and 2 contain inactive and active Caspase, respectively.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Western-Blot-NB500-210-img0004.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate buffer.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Immunohistochemistry-Paraffin-NB500-210-img0001.jpg)
![Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Simple-Western-NB300-270-img0009.jpg "Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Lysates of the human RPE cell lines ARPE-19 (lane 1) and D407 (lane 2), the mouse RPE cell line B6-RPE07 (lane 3), mouse primary RPE (lane 4) and mouse brain lysates (lane 5) were harvested and analyzed by immunoblot assay with DJ-1 antibody. The DJ-1 signal varied in intensity in each RPE cell culture. Each lane contained 20 ug of protein. Protein loads were confirmed in replicate blots probed with GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0014.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB300-270] - Representative confocal micrographs of ARPE-19 monolayers plated on Transwells(R) and labeled with antibodies to DJ-1 (G,J) and COX IV (H,K). Under baseline conditions, there is very little colocalization between DJ-1 and COX IV, as observed in overlaid images (I); DJ-1 is mostly distributed through the cytoplasm (arrows). Upon oxidative stress induced by incubation with 400 uM H2O2 for 1 hr, DJ-1 staining is increased (arrowheads) in ARPE-19 (J) cultures. In cultures treated with H2O2 some DJ-1 re-distributed to mitochondria (arrowheads) and displayed significant colocalization with COX IV in overlaid images (L). Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-270-img0015.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - ARPE-19 and D407 monolayers were treated with increasing concentrations (0 to 800 uM) of H2O2 for 18 hrs (B), harvested, and analyzed by immunoblot assay with DJ-1 antibody (upper panel). Each lane contained 20 ug of protein. Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). A representative Western is shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY license](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0013.jpg)
![Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB300-270] - Park7(DJ-1) detected in paraffin embedded human cortex.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Immunohistochemistry-Paraffin-NB300-270-img0007.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Human HEK293 cells and mouse brain lysates.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0005.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY PARK7 cDNA (lane 2) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Park7(DJ-1).](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0008.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Presence of oxDJ-1 in RPE cells subjected to oxidative stress.ARPE-19 monolayers were treated with increasing concentrations (0 to 800 µM) of H2O2 for 1 hr (A) & 18 hs (B), harvested, & analyzed by immunoblot assay with oxDJ-1 antibody (upper panel). Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). Each lane contained 20 µg of protein. A dose response is observed when cells are exposed to increasing concentrations of H2O2 for 1 h (A, lanes 1 to 6) & 18 hrs (B, lanes 7 to 12). Confocal immunofluorescence staining of baseline ARPE-19 cultures (C) fixed before extraction with Triton X-100 & labeling with oxDJ-1 antibodies revealed absence of oxDJ-1. However, oxDJ-1 is observed in the cytoplasm (arrows) & perinuclear area (arrowheads) of RPE cells exposure to 400 µM H2O2 for 1 h (D) & 18 hrs (E). Cell nuclei were labeled with TO-PRO-3. Scale bar = 20 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23844142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-270_rabbit-polyclonal-park7-dj-1-antibody-31020241621815.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Presence of oxDJ-1 in RPE cells subjected to oxidative stress.ARPE-19 monolayers were treated with increasing concentrations (0 to 800 µM) of H2O2 for 1 hr (A) & 18 hs (B), harvested, & analyzed by immunoblot assay with oxDJ-1 antibody (upper panel). Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). Each lane contained 20 µg of protein. A dose response is observed when cells are exposed to increasing concentrations of H2O2 for 1 h (A, lanes 1 to 6) & 18 hrs (B, lanes 7 to 12). Confocal immunofluorescence staining of baseline ARPE-19 cultures (C) fixed before extraction with Triton X-100 & labeling with oxDJ-1 antibodies revealed absence of oxDJ-1. However, oxDJ-1 is observed in the cytoplasm (arrows) & perinuclear area (arrowheads) of RPE cells exposure to 400 µM H2O2 for 1 h (D) & 18 hrs (E). Cell nuclei were labeled with TO-PRO-3. Scale bar = 20 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23844142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-270_rabbit-polyclonal-park7-dj-1-antibody-3102024162185.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Presence of oxDJ-1 in RPE cells subjected to oxidative stress.ARPE-19 monolayers were treated with increasing concentrations (0 to 800 µM) of H2O2 for 1 hr (A) & 18 hs (B), harvested, & analyzed by immunoblot assay with oxDJ-1 antibody (upper panel). Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). Each lane contained 20 µg of protein. A dose response is observed when cells are exposed to increasing concentrations of H2O2 for 1 h (A, lanes 1 to 6) & 18 hrs (B, lanes 7 to 12). Confocal immunofluorescence staining of baseline ARPE-19 cultures (C) fixed before extraction with Triton X-100 & labeling with oxDJ-1 antibodies revealed absence of oxDJ-1. However, oxDJ-1 is observed in the cytoplasm (arrows) & perinuclear area (arrowheads) of RPE cells exposure to 400 µM H2O2 for 1 h (D) & 18 hrs (E). Cell nuclei were labeled with TO-PRO-3. Scale bar = 20 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23844142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-270_rabbit-polyclonal-park7-dj-1-antibody-310202416212344.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - Human kidney distal tubules stained with E-CAD antibody. Secondary antibody: Donkey anti-Mouse Alexa Fluor 555. Imaging with a 40x objective from Zeiss Observer microscope with Apotome2. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunohistochemistry-Paraffin-NBP2-19051-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon PDK1 knockdown in Colo205 and HT29 cells using E cadherin (NBP2-19051), N cadherin (NBP1-48309) and B-actin antibody (NB600-501). The corresponding secondary antibodies used were either goat anti-rabbit IgG-HRP (NB7160) or goat anti-mouse IgG-HRP (NB7539). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0013.jpg)
![Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of E-Cadherin in LNCAP (1), A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - ICC staining of E-Cadherin in human colon cancer sphere. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunocytochemistry-Immunofluorescence-NBP2-19051-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Mitochondrial fission regulates migration and invasion in BRAF^V600E CRC cells through glucose metabolic reprograming. Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAF^V600E cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - Human colonic tissue was stained with E-Cadherin antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunocytochemistry-Immunofluorescence-NBP2-19051-img0009.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin antibody in mouse colon cancer tissue. Antibody dilution 1:500. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunohistochemistry-Paraffin-NBP2-19051-img0012.jpg)
![Flow Cytometry: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of Hela cells using E-Cadherin mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Flow-Cytometry-NBP2-19051-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma, 6nM TNF alpha, and 3nM IL1 beta). Expression of Snail, E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0015.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin in gastric cancer tissues (left) and lung cancer tissues (right) with DAB staining.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunohistochemistry-Paraffin-NBP2-19051-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0011.jpg "Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).")
![Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunohistochemistry-Paraffin-NBP1-47386-img0014.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Flow-Cytometry-NBP1-47386-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD44 Antibody [8E2F3] conjugated to DyLight 550 (NBP1-47386R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0018.jpg)
![Western Blot: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - Generation and characterization of immortalized primary keratinocytes (IPKs) from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. Western blot analysis of pSTAT3, STAT3, and CD44 (NBP1-47386) in primary keratinocytes and IPKs from both genotypes. The A431 cells were used as positive controls. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29955047/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-BSA-Free-Western-Blot-NBP1-47386-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD44 Antibody [8E2F3] conjugated to Alexa Fluor 647 (NBP1-47386AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0019.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Flow-Cytometry-NBP1-47386-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0010.jpg)
![Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Flow-Intracellular-NBP1-47386-img0016.jpg)
![Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - HAP1 lysates were prepared and immunoprecipitation was performed using 1.0 ug of the CD44 Antibody (NBP1-47386) pre-coupled to either protein G or protein A Sepharose beads. Ability of the antibodies to capture CD44 antigen was first assessed by comparing the level of CD44 antigen from the starting material (SM) to its level remaining in the unbound fractions (UB). Anti-CD44 antigen at 1/2000 was used for each immunoblot. Immunoprecipitate for CD44 Antibody (NBP1-47386) that showed depleted CD44 antigen in the UB can be seen. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-BSA-Free-Immunoprecipitation-NBP1-47386-img0020.jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).](http://images.novusbio.com/fullsize/COX-2-Antibody-Immunohistochemistry-Paraffin-NB100-689-img0010.jpg "Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).")
![Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image provided through customer review.](http://images.novusbio.com/fullsize/COX-2-Antibody-Simple-Western-NB100-689-img0003.jpg)
![Immunohistochemistry Free-Floating: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse GFAP mAb, dilution 1:1,000 (Green), costained with rabbit neurofilament NF-L pAb, dilution 1:2,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with antibodies. The GFAP antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Immunohistochemistry-Free-Floating-NBP1-05197-img0007.jpg "Immunohistochemistry Free-Floating: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse GFAP mAb, dilution 1:1,000 (Green), costained with rabbit neurofilament NF-L pAb, dilution 1:2,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with antibodies. The GFAP antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.")
![Simple Western: GFAP Antibody (5C10) [NBP1-05197] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Simple-Western-NBP1-05197-img0004.jpg)
![Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - The immunofluorescence of TLR7 recognized by Alexa 488, green (NBP2-24906). GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Immunohistochemistry-NBP1-05197-img0008.jpg)
![Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse mAb to GFAP, NBP1-05197, dilution 1:1,000, in green, costained with rabbit pAb to neurofilament NF-L, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-05197 antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Immunocytochemistry-Immunofluorescence-NBP1-05197-img0005.jpg)
![Western Blot: GFAP Antibody (5C10) [NBP1-05197] - Analysis of whole tissue lysates using mouse mAb to GFAP, NBP1-05197, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 50kDa corresponds to the GFAP protein.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Western-Blot-NBP1-05197-img0006.jpg)