| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-202SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77686SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1992SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-37235SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27103SS | |||
| Azide and BSA Free | NBP2-80749 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22119SS | |||
| Azide and BSA Free | NBP2-80814 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-231SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-56893SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-402SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-556-0.025ml | |||
![Western Blot: STEP Antibody (23E5) [NB300-202] - Analysis of STEP protein in striatal rat protein homogenates](http://images.novusbio.com/fullsize/STEP-Antibody-23E5-Western-Blot-NB300-202-img0002.jpg "Western Blot: STEP Antibody (23E5) [NB300-202] - Analysis of STEP protein in striatal rat protein homogenates")
![Immunohistochemistry-Paraffin: STEP Antibody (23E5) [NB300-202] - STEP was detected in immersion fixed paraffin-embedded sections of human brain basal ganglia using Mouse Anti-Human STEP (23E5) Monoclonal Antibody (Catalog # NB300-202) at 1:100 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in neurons.](http://images.novusbio.com/fullsize/STEP-Antibody-23E5-Immunohistochemistry-Paraffin-NB300-202-img0003.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - IHC analysis of formalin fixed paraffin embedded tissue section from hippocampus of mouse brain with NeuN antibody at 1:200 dilution. The antibody generated a strong nuclear with weak cytoplasmic staining in NeuN reactive cells.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunohistochemistry-Paraffin-NBP1-77686-img0007.jpg "Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - IHC analysis of formalin fixed paraffin embedded tissue section from hippocampus of mouse brain with NeuN antibody at 1:200 dilution. The antibody generated a strong nuclear with weak cytoplasmic staining in NeuN reactive cells.")
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - JAK3 colocalized with neurons labeled with NeuN. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fneur.2017.00363/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunohistochemistry-Paraffin-NBP1-77686-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP1-77686] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-RBFOX3/NeuN Antibody NBP1-77686 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77686-img0013.jpg)
![Western Blot: RBFOX3/NeuN Antibody [NBP1-77686] - Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Western-Blot-NBP1-77686-img0012.jpg)
![Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on Hek293 cells with NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Flow-Cytometry-NBP1-77686-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP1-77686] - NeuN antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77686-img0005.jpg)
![Immunohistochemistry: RBFOX3/NeuN Antibody [NBP1-77686] - Analysis of NeuN in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunohistochemistry-NBP1-77686-img0008.jpg)
![Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on U2OS cells with RBFOX3/NeuN Antibody NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Flow-Cytometry-NBP1-77686-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody - BSA Free [NBP1-77686] - Double immunofluorescence indicates that NF-kappa B & CCL5 were co-localized with microglia, astrocytes, & neurons in the ipsilateral L4–5 spinal cord on day 7 after CCI.NF-kappa B (green) colocalizes with Iba-1, GFAP, & NeuN (red) in laminae II–III of the superficial dorsal horn (A). CCL5 (red) co-localizes with Iba-1, GFAP, & NeuN (green) in the medial superficial dorsal horn (laminae II–III) (B). NF-kappa B (green) co-localizes with CCL5 (red) in laminae II–IV of the superficial dorsal horn following CCI on day 7 in the sham & CCI groups in the ipsilateral L4–5 spinal cord (C). Two single stained images (yellow) were merged. Scale = 100 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115120), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77686_rabbit-polyclonal-rbfox3-neun-antibody-31020241538241.jpg)
![Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody - BSA Free [NBP1-77686] - (A) Representative Western blots depicting phosphorylated JAK3 (pJAK3), total JAK3, & corresponding beta -actin levels in the ipsilateral (CXI) versus the contralateral (CXC) cortex at 24 h in sham & mice subjected to permanent middle cerebral artery occlusion (pMCAO). (B,C) Quantified pJAK3 & JAK3 values normalized to beta -actin in the cortex of sham & mice subjected to stroke. Both pJAK3 & JAK3 are significantly increased in the ipsilateral cortex of stroked animals (*P < 0.05 & **P < 0.01 with respect to sham ipsilateral/contralateral & stroke contralateral; sham, n = 5; stroke, n = 13). Data were analyzed using a two-way ANOVA (ipsilateral/contralateral or sham/stroke) with Bonferroni post-test. (D) Diagram showing peri-infarct brain region from which images were taken (n = 3). Scale bar is 10 µm, & magnification is 60×. (E) JAK3 colocalized with microglia/macrophages labeled with Iba-1. (F) JAK3 colocalized with neurons labeled with NeuN. (G) JAK3 colocalized with endothelial cells labeled with CD31. (H) JAK3 is not colocalized with astrocytes labeled with GFAP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28790974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77686_rabbit-polyclonal-rbfox3-neun-antibody-310202416175267.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, and protein expression of oncogenes and tumor suppressive genes in 1alpha(OH)ase -/- mice. Mice from each group were treated as described in Figure 1. Representative micrographs of skin sections stained immunohistochemically for SOD2 and gamma-H2AX. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.12951), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-NB100-1992-img0013.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, and protein expression of oncogenes and tumor suppressive genes in 1alpha(OH)ase -/- mice. Mice from each group were treated as described in Figure 1. Representative micrographs of skin sections stained immunohistochemically for SOD2 and gamma-H2AX. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.12951), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1992-img0010.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Western blot analysis of Rat Tissue lysates showing detection of SOD2/Mn-SOD protein using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NB100-1992-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1992-img0012.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - Immunohistochemistry analysis using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-NB100-1992-img0017.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-31020241555245.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-3102024168392.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-31020241553748.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - CAV-1 preserves mitochondrial respiratory function through regulation of OXPHOS expression.(A) OXPHOS complex subunits were detected through western blotting with appropriate antibodies. CAV-1 treatment significantly restored hypercholesterolemia-associated increased ETC complex I–V proteins levels. (B) Percentage of the OXPHOS complex (I–V) band intensities is presented in the graph. (C) Representative immunoblot displaying levels of CAV-1, CyPA, mitochondrial biogenesis markers, & antioxidant enzymes in the NC, HC, & HCAV-1 groups. (D) Columns represent average values over three independent experiments. The density for the NC group was set at 1; * & † are significantly different for the NC & HC groups, respectively, at P < 0.05. beta -actin was used as a loading control. Values are means ± SD. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154210), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-3102024155523.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-310202415541978.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-310202415535131.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-3102024165716.jpg)
![Western Blot: RelA/NFkB p65 Antibody [NB100-2176] - Human glomerular endothelial cell lysates, time-response treatment with 10ng/ml TNF-alpha. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-Western-Blot-NB100-2176-img0006.jpg "Western Blot: RelA/NFkB p65 Antibody [NB100-2176] - Human glomerular endothelial cell lysates, time-response treatment with 10ng/ml TNF-alpha. This image was submitted via customer Review.")
![Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody [NB100-2176] - Staining of formalin-fixed, paraffin-embedded human DLBCL showing nuclear expression of NFkB p65 in the tumor cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-Immunohistochemistry-Paraffin-NB100-2176-img0004.jpg)
![Western Blot: RelA/NFkB p65 Antibody [NB100-2176] - Analysis of NFkB p65 expression in A431 (A) and HeLa (B) whole cell lysates.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-Western-Blot-NB100-2176-img0005.jpg)
![Immunohistochemistry-Paraffin: Rabbit Polyclonal RelA/NFkB p65 Antibody [NB100-2176] - RelA/NFkB p65 IHC (brown) in the mice liver tissue; bars, 200 μm. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-2176_rabbit-polyclonal-rela-nfkb-p65-antibody-immunohistochemistry-paraffin-1111202410307..jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit Polyclonal RelA/NFkB p65 Antibody [NB100-2176] - Mice hepatocytes stained for RelA/NFkB p65. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-2176_rabbit-polyclonal-rela-nfkb-p65-antibody-immunocytochemistry-immunofluorescence-2511202410544..png)
![Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunohistochemistry-Paraffin-NB110-37235-img0012.jpg)
![Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 at 0.5 ug/mL.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NB110-37235-img0008.jpg)
![Flow Cytometry: SOX2 Antibody [NB110-37235] - An intracellular stain was performed on A549 with NB110-37235 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylgiht 550.](http://images.novusbio.com/fullsize/SOX2-Antibody-Flow-Cytometry-NB110-37235-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SOX2 Antibody [NB110-37235] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NB110-37235-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SOX2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-37235-img0019.jpg)
![Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235] - An intracellular stain was performed on U-251 MG cells with SOX2 Antibody NB110-37235 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/SOX2-Antibody---BSA-Free-Flow-Cytometry-NB110-37235-img0020.jpg)
![Western Blot: SOX2 Antibody [NB110-37235] - Expression of pluripotency factor Sox2 in human head and neck cancer cell lines FaDu and HN5. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NB110-37235-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - Staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-37235-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-SOX2 (NB110-37235) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-37235-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SOX2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-37235-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (13H12) [NBP2-27103] - GAPDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunocytochemistry-Immunofluorescence-NBP2-27103-img0013.jpg "Immunocytochemistry/Immunofluorescence: GAPDH Antibody (13H12) [NBP2-27103] - GAPDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A dilution of 1:10 was used. Image objective 40x.")
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein (theoretical molecular weight: 36 kDa) in HeLa cells lysate using GAPDH antibody (clone 13H12) in (A) the absence and (B) the presence of immunizing peptide.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0001.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human rectal carcinoma tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0011.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human breast tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0008.jpg)
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - Characterization of lncR492.(B) Northern blot of lncR494 using increasing amounts of loaded total. Black arrow signifies lncR492-specific signal at approximately 1400 bp. A probe targeting Gapdh mRNA served as the loading control. (D) RT-PCR analysis of lncR492 and Gapdh expression. RNA extract was treated with a 5'-phosphate-dependent exonuclease, resulting in a degradation of f.ex. ribosomal RNA.Citation: Winzi M, Casas Vila N, Paszkowski-Rogacz M, Ding L, Noack S, Theis M, et al. (2018) The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells. PLoS ONE 13(1): e0191682. //doi.org/10.1371/journal.pone.0191682](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0017.jpg)
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - Serum from trauma patients induces intrinsic apoptosis resistance by up-regulating AAT expression in neutrophils. AAT protein expression was analyzed in neutrophils after 18 h of culture by western blot analysis. Relative expression was quantified vs. GAPDH expression. One representative blot of five independent experiments is depicted. **p<0.01 (one-way ANOVA with Newman keuls post-hoc test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0177450), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0018.jpg)
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein (theoretical molecular weight 36 kDa) in lysates of Mouse cell lines (A) NIH 3T3 (B) RAW 264.7 using GAPDH antibody (clone 13H12) at a concentration of 0.25 ug/ml.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0005.jpg)
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - analysis of GAPDH in HeLa and HEK 293 cells (25ug/lane) using anti-GAPDH antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0014.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human stomach tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0007.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human colon tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0009.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0010.jpg)
![Simple Western: GAPDH Antibody (13H12) [NBP2-27103] - GAPDH/G3PDH Antibody (13H12) [NBP2-27103] - Simple Western lane view shows a specific band for GAPDH in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Simple-Western-NBP2-27103-img0015.jpg)
![Western Blot: GAPDH Antibody (13H12) - BSA Free [NBP2-27103] - Effect of AAT on Mcl-1 phosphorylation, the activity of MAP kinases & caspases.Neutrophils from healthy volunteers (2.5 × 106/ml) were cultured in medium supplemented with patient serum (3 mg protein/ml; 1%) & those containing low levels of AAT (AAT-reduce serum; 1%) in the presence of STS (0.2 μM). After 3 h, the expression of pMcl-1 (A, n = 7), pAkt (B, n = 8) & pERK1/2 (C, n = 10) were analyzed by western blot. Expression levels of the phosphorylated proteins were normalized to that of the unphosphorylated forms. GAPDH was used as loading control. One representative blot is displayed. *p<0.05; **p<0.01; n.s. = not significant. D. After 4 h of incubation the activities of caspase-9 & caspase-3/-7 were quantified. Results are presented as means ± SEM of eight independent experiments. No significant differences were found (one-way ANOVA with Newman keuls post-hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28493974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27103_mouse-monoclonal-gapdh-antibody-13h12-imgenex-img-6665a-25-31020241612222.jpg)
![Western Blot: GAPDH Antibody (13H12) - BSA Free [NBP2-27103] - Effect of AAT on Mcl-1 phosphorylation, the activity of MAP kinases & caspases.Neutrophils from healthy volunteers (2.5 × 106/ml) were cultured in medium supplemented with patient serum (3 mg protein/ml; 1%) & those containing low levels of AAT (AAT-reduce serum; 1%) in the presence of STS (0.2 μM). After 3 h, the expression of pMcl-1 (A, n = 7), pAkt (B, n = 8) & pERK1/2 (C, n = 10) were analyzed by western blot. Expression levels of the phosphorylated proteins were normalized to that of the unphosphorylated forms. GAPDH was used as loading control. One representative blot is displayed. *p<0.05; **p<0.01; n.s. = not significant. D. After 4 h of incubation the activities of caspase-9 & caspase-3/-7 were quantified. Results are presented as means ± SEM of eight independent experiments. No significant differences were found (one-way ANOVA with Newman keuls post-hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28493974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27103_mouse-monoclonal-gapdh-antibody-13h12-imgenex-img-6665a-25-310202416114762.jpg)
![Western Blot: GAPDH Antibody (13H12) - BSA Free [NBP2-27103] - Effect of AAT on Mcl-1 phosphorylation, the activity of MAP kinases & caspases.Neutrophils from healthy volunteers (2.5 × 106/ml) were cultured in medium supplemented with patient serum (3 mg protein/ml; 1%) & those containing low levels of AAT (AAT-reduce serum; 1%) in the presence of STS (0.2 μM). After 3 h, the expression of pMcl-1 (A, n = 7), pAkt (B, n = 8) & pERK1/2 (C, n = 10) were analyzed by western blot. Expression levels of the phosphorylated proteins were normalized to that of the unphosphorylated forms. GAPDH was used as loading control. One representative blot is displayed. *p<0.05; **p<0.01; n.s. = not significant. D. After 4 h of incubation the activities of caspase-9 & caspase-3/-7 were quantified. Results are presented as means ± SEM of eight independent experiments. No significant differences were found (one-way ANOVA with Newman keuls post-hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28493974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27103_mouse-monoclonal-gapdh-antibody-13h12-imgenex-img-6665a-25-310202416121949.jpg)
![Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Western-Blot-NBP2-22119-img0007.jpg "Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.")
![Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-22119-img0006.jpg)
![Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin (APC).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Flow-Cytometry-NBP2-22119-img0011.jpg)
![Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin (PE).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Flow-Cytometry-NBP2-22119-img0010.jpg)
![Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Western-Blot-NBP2-22119-img0002.jpg)
![Flow Cytometry: iNOS Antibody (4E5) - BSA Free [NBP2-22119] - An intracellular stain was performed on NIH3T3 cells with iNOS Antibody (4E5) NBP2-22119 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-BSA-Free-Flow-Cytometry-NBP2-22119-img0013.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-22119-img0009.jpg)
![Flow Cytometry: iNOS Antibody (4E5) - BSA Free [NBP2-22119] - An intracellular stain was performed on Caco-2 cells with iNOS Antibody (4E5) NBP2-22119 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-BSA-Free-Flow-Cytometry-NBP2-22119-img0012.jpg)
![ELISA: iNOS Antibody (4E5) [NBP2-22119] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/iNOS-Antibody-4E5-ELISA-NBP2-22119-img0001.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody [NB600-1235] - Formalin fixed paraffin embedded human tonsil stained with Caspase-3 Antibody (NB600-1235).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Immunohistochemistry-Paraffin-NB600-1235-img0003.jpg "Immunohistochemistry-Paraffin: Caspase-3 Antibody [NB600-1235] - Formalin fixed paraffin embedded human tonsil stained with Caspase-3 Antibody (NB600-1235).")
![Immunohistochemistry-Paraffin: Caspase-3 Antibody [NB600-1235] - Human tonsil.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Immunohistochemistry-Paraffin-NB600-1235-img0002.jpg)
![Immunohistochemistry: Caspase-3 Antibody [NB600-1235] - Stained sections of fish intestine and liver of the control group showed negative staining reactions in all the examined parts of the intestinal and hepatic tissue (black and red arrows) against caspase-3 (NB600-1235) and TNF-alpha (NBP2-61611) antibodies. Nearly 10% of hepatic portal cells reacted positively to caspase-3. Scale bars = 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35624801/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb600-1235_rabbit-polyclonal-caspase-3-antibody-imgenex-img-80432-20920231721154.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 (NB300-229) on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-231-img0014.jpg "Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 (NB300-229) on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.")
![Immunohistochemistry: SCP3/SYCP3 Antibody [NB300-231] - Punctate staining of murine SCP3 in mouse ovary using NB300-231.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-NB300-231-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Distinct forms of ubiquitin regulation by RNF8 and SCML2. (C-F) Immunostaining on meiotic chromosome spreads of FK2 and SYCP3 antibodies. Dotted circles are sex chromosomes and Scale bar are 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages). Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of D27C4 and SYCP3 on meiotic chromosome spreads. Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages).Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A) Immunostaining of D27C4 and SYCP3 on wild-type meiotic chromosome spreads. Representative images are shown for each category through the early pachytene stage for spermatocytes from 9 independent mice. (C) Immunostaining of SYCP3 and D27C4 on Mdc1-KO meiotic chromosome spreads. Representative images are shown through the early pachytene stage for spermatocytes from 3 independent Mdc1-KO mice. (A and C) Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages).Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of E6C5 and SYCP3 on meiotic chromosome spreads. Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model.Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A, B) Immunostaining of SCML2 and SYCP3 on meiotic chromosome spreads. Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model.Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of H3K4me2 and SYCP3 on meiotic chromosome spreads. Dotted circles: sex chromosomes. (E) Regulation of H3K4me2. (F, G) Immunostaining of H3K4me2 and SYCP3 on wild-type and Scml2-KO meiotic chromosome spreads. All: Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages).Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of H3K27ac and SYCP3 on meiotic chromosome spreads. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages). (E) Model of the regulation of H3K27ac. (F) Immunostaining of SYCP3 and H3K27ac on wild-type and Rnf8-KO round spermatids. At least 30 round spermatids shown from at least 3 independent mice. All: Dotted circles indicate sex chromosomes and scale bar is 10 micrometers.Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Mouse spermatocytes stained with biotin-conjugated anti-SYCP3 detected with streptavidin-Alexa Fluor 594 (left panel), streptavidin Alexa Fluor 488 (right panel). Image from verified customer review.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-231-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - SCP3 labeled in mouse pachytene preparation (red), using NB 300-231. CDK2 staining, near teleomeres, is also present (green).](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-231-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - CXXC1 is expressed in the spermatocytes in the presence or absence of PRDM9.(A) Immunofluorescence staining for CXXC1 & PRDM9 in adult B6, 14-dpp B6 & 14-dpp Prdm9-/- seminiferous tubule cross sections. Green, CXXC1; red, PRDM9; blue, DAPI. SC, Sertoli cell; Z, zygonema; P, pachynema; RS, round spermatid. Scale bars, first 4 columns: 50 μm, last column: 10 μm. (B) Immunofluorescence staining for CXXC1 & PRDM9 on chromosome spreads from adult B6. Green, SYCP3; orange, PRDM9; magenta, CXXC1. Scale bars, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202415392592.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - RNF8 is required for polyubiquitination of the sex chromosomes: Immunostaining with E6C5 antibody, which recognizes polyubiquitination.(A-D) Immunostaining of SYCP3 & E6C5 on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. (E) Schematic of ubiquitin targets recognized by the E6C5 antibody in each mouse model. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007233), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202415291958.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - RNF8-dependent H2AK119ub is removed by SCML2: Immunostaining with D27C4 antibody, which recognizes H2AK119ub.(A-D) Immunostaining of SYCP3 & D27C4 (H2AK119ub) on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. The intensity of immunostaining is quantified by densitometry across the indicated path (‘ to”) & plotted in a relative intensity range of 0–1, which is normalized among all images in this figure, Fig 3 & S2 Fig. (E) Updated model of distinct forms of regulation of ubiquitination by RNF8 & SCML2, including RNF8-dependent H2AK119ub, which is removed by SCML2 based on data in this figure. (F) Schematic of ubiquitin targets recognized by the D27C4 (H2AK119ub) antibody in each mouse model. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007233), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416205194.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Distinct forms of ubiquitin regulation by RNF8 & SCML2: Immunostaining with FK2 antibody, which recognizes both mono- & polyubiquitinated conjugates.(A) Schematic of spermatogenesis. (B) Model of distinct forms of regulation of ubiquitination by RNF8 & SCML2. (C-F) Immunostaining of SYCP3 & FK2 on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. The intensity of immunostaining is quantified by densitometry across the indicated path (‘ to”) & plotted in a relative intensity range of 0–1, which is normalized among the samples at the same stage. (G) Schematic of ubiquitin targets recognized by the FK2 antibody in each mouse model. A pink circle denotes RNF8-dependent ubiquitination & an orange circle denotes events mediated by a different E3 ubiquitin ligase. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007233), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416212367.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - No major meiotic DSB repair or chromosome synapsis defects are observed in Cxxc1 CKO testis.(A) Immunostaining of SYCP3 & gamma H2AX on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; magenta, gamma H2AX. Scale bar, 10 μm. (B) Spermatocyte stage proportion in adult Cxxc1 het (n = 1,062 from two individuals) & CKO (n = 1,105 from two individuals) spermatocytes based on SYCP3/SYCP1/ gamma H2AX staining. p = 0.7 by Chi-square test. (C) Immunostaining of SYCP3 & SYCP1 on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; orange, SYCP1. Scale bar, 10 μm. (D) Crossover number measured by MLH1 staining on chromosome spreads of adult Cxxc1 het & CKO spermatocytes. Left, magenta, SYCP3; green, MLH1. Scale bar, 10 μm. Right, number of MLH1 foci per late pachynema in Cxxc1 het (n = 32 from two individuals) & CKO (n = 33 from two individuals). Bars represent mean ± SD. p = 0.4 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-3102024162188.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Meiotic spreads show deletion of Dicer1 disrupts progression of meiosis I.(A) It was possible to identify the 5 sub-stages of meiosis I by combining the reactivity patterns of two antibodies against synaptonemal complex protein 3 (SYCP3) & gamma H2AX. SYCP3 is a protein essential for synapsis of homologous chromosomes & gamma H2AX localizes to double-strand breaks & XY bodies during meiosis. A total of 200 spreads were counted in testis cell preparations from WT & GCKO mice at P22. (B) Chi square analysis shows GCKO testes contained significantly higher numbers of germ cell spreads at the leptotene & zygotene stages of meiosis I & fewer spreads at pachytene, diplotene & metaphase I stages (P<0.001), suggesting that the loss of Dicer1 lead to disruptions in progression through meiosis I. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23056286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416212316.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - PRDM9 expression & catalytic function are not impaired in Cxxc1 CKO.(A) Immunostaining of PRDM9 shows unchanged pattern in adult CKO seminiferous tubules compared to the heterozygous control. Red, PRDM9; grey, DAPI. Scale bar, 20 μm. (B) PRDM9 & SYCP3 expression patterns are not changed in CXXC1 CKO chromosome spreads compared to the heterozygous control. Co-immunostaining of CXXC1 & PRDM9 on chromosome spreads from adult Cxxc1 het & CKO mice. Green, SYCP3; orange, PRDM9; magenta, CXXC1. First 4 rows, het control; last 4 rows, Cxxc1 CKO. Scale bar, 10 μm. (C) Meiosis progression occurs normally in Cxxc1 CKO testes. Immunostaining of H3K4me3 in adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; magenta, H3K4me3. Scale bar, 10 μm. (D) Testis-specific gene expression is not changed in Cxxc1 CKO testes. H3K4me3 ChIP-qPCR with chromatin isolated form Cxxc1 het & CKO mice. Promoter regions form Actinb & Sycp3, Dom2 hotspots PbxI & Fcgr4 were amplified. Cst hotspot HlxI was used as a negative control. Bars present mean ± SD of three biological replicates. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416205179.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - DSB number is not affected in Cxxc1 CKO.The DSB number was determined by three markers reflecting different stages of their processing. (A) DMC1 staining on Cxxc1 control & CKO chromosome spread. Bottom panels, distribution plot of DMC1 foci in early zygotene (n = 22 in het, n = 34 in CKO), late zygotene (n = 29 in het, n = 36 in CKO) & pachytene (n = 36 in het, n = 26 in CKO) spermatocytes. (B) RAD51 staining on Cxxc1 control & CKO chromosome spread. Bottom panels, distribution plot of RAD51 foci in early zygotene (n = 22 in het, n = 13 in CKO), late zygotene (n = 24 in het, n = 11 in CKO) & pachytene (n = 52 in het, n = 36 in CKO) spermatocytes. (C) RPA staining on Cxxc1 control & CKO chromosome spread. Bottom panels, distribution plot of RPA foci in early zygotene (n = 35 in het, n = 43 in CKO), late zygotene (n = 33 in het, n = 28 in CKO) & pachytene (n = 89 in het, n = 92 in CKO) spermatocytes. For A-C, two individuals per genotype were measured. Bars represent mean ± SD. Scale bar, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416203720.jpg)
![Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. (1) siRNA ATG9A knockdown and (2) wildtype ATG9A.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Western-Blot-NB110-56893-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. (1) siRNA ATG9A knockdown and (2) wildtype ATG9A.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody [NB110-56893] - After stimulation with the NOD1 ligand, macrophages increased their expression of autophagy proteins Atg9, LC3 and IRGM. Alveolar macrophages (AMs), monocyte-derived macrophages (MDMs) and monocytes (MNs) were incubated in the presence of 5 ug/ml of Tri-DAP for 24 h. Cells were pre-incubated with Rip2/p38 inhibitor SB203580 (SB) for 30 min prior to Tri-DAP stimulation to block NOD1-mediated responses, as indicated. Atg9 and LC3 proteins were measured in the cytosolic fractions by western blot analysis. Image collected and cropped by CiteAb from the following publication (//bmcpulmmed.biomedcentral.com/articles/10.1186/1471-2466-14-152), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Western-Blot-NB110-56893-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody [NB110-56893] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-ATG9A at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-56893-img0008.jpg)
![Immunohistochemistry: ATG9A Antibody [NB110-56893] - Staining in mouse intestine.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunohistochemistry-NB110-56893-img0005.jpg)
![Flow Cytometry: ATG9A Antibody [NB110-56893] - An intracellular stain was performed on Hela Cells with NB110-56893 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).](http://images.novusbio.com/fullsize/ATG9A-Antibody-Flow-Cytometry-NB110-56893-img0007.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of chloroquine, spautin-1 & MG132 on ATG protein levels in A549 cells. (A) A549 cells were treated with 50 μM chloroquine, 10 μM spautin-1 or 5 μM MG132 in the presence or absence of 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3, mean±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415371977.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - CD133 promoted glucose uptake.A. Levels of glucose uptake in Huh-7con & Huh-7sh133 cells. Mean fluorescence intensity of 2-NBDG uptake was measured by FASC analysis after incubating for 6 h. B. Time course of 2-NBDG uptake in these two cell lines. Statistical significance indicated as *p<0.05; **p<0.01. C. CD133 or empty vector was transfected respectively into LM3 cells. Glucose uptake was measured at the indicated times upon the medium added with 100 µM 2-NBDG. *p<0.05. D. Detection of relative activities of signaling pathways in Huh-7con & Huh-7sh133 cells by Western blotting. con: Huh-7con cells; sh: Huh-7sh133 cells. E. Detection of autophagic genes in Huh-7con & Huh-7sh133 cells by Western blotting. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437259), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415382433.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of TGF beta 1 on ATG protein levels & LC3B lipidation in A549 cells. (A) A549 cells were treated with 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415384151.jpg)
![Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody [NB100-402] - CTR1 antibody was tested in NIH-3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-402-img0004.jpg "Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody [NB100-402] - CTR1 antibody was tested in NIH-3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunohistochemistry: SLC31A1/CTR1 Antibody [NB100-402] - Panel 1: human CTR1 staining of breast cancer tissue. Panel 2: human CTR1-antigen competition in breast cancer tissue.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-NB100-402-img0002.jpg)
![Immunohistochemistry: SLC31A1/CTR1 Antibody [NB100-402] - Left panel: Antibody staining of hCTR1 overexpressing cell line. Right panel: Antibody plus peptide competition staining.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-NB100-402-img0003.jpg)
![Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Western-Blot-NB300-556-img0020.jpg "Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.")
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures. H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) and (I) showing postsynaptic labeling of GluA1. Scale bar (valid for all images): 10 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140868), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0025.jpg)
![Immunohistochemistry-Paraffin: PSD-95 Antibody (6G6-1C9) [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 Double labeling with antibodies against syntaxin-1 (D) and synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunohistochemistry-Paraffin-NB300-556-img0024.jpg)
![Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Flow-Cytometry-NB300-556-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in HeLa cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in U251 glioma cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in C6 glioma cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of Post Synaptic Density 95kD protein (PSD95, green) in cultured primary cortical neurons. Primary cortical neurons are isolated and cultured using the Primary Neuron Isolation Kit. At day 28, neurons were fixed with 4% paraformaldehyde, permeablilized with 0.1% triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were probed with a PSD95 monoclonal antibody at a dilution of 1:500 for 2 hours at room temperature or overnight at 4C, washed with HBSS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Mixed 3 week old rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). The antibody labels small punctae along neuronal processes as expected. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0021.jpg)
![Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in Neuro-2a cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Flow-Cytometry-NB300-556-img0012.jpg)
![Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in SH-SY5Y cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Flow-Cytometry-NB300-556-img0013.jpg)
![Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in U87-MG cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Flow-Cytometry-NB300-556-img0014.jpg)
![Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 (A) & anti-Tuj1 (B) demonstrates that syntaxin-1 is located along the dendrites & gives characteristic punctate labeling (C). Double labeling with antibodies against syntaxin-1 (D) & synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Double labeling for syntaxin-1 (G) & PSD-95 (H) shows partial colocalization postsynaptic (I). Scale bars: 10 μm. (J–L) Western blot analysis of synaptosomes (Syn), synaptic cytosolic fraction (Cy), active zone (AZ) & post synaptic density (PSD). Labeled with anti-synaptophysin (J) anti-PSD95 (K) & anti-syntaxin-1 (L). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-556_mouse-monoclonal-psd-95-antibody-6g6-1c9-310202415392516.jpg)
![Western Blot: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 (A) & anti-Tuj1 (B) demonstrates that syntaxin-1 is located along the dendrites & gives characteristic punctate labeling (C). Double labeling with antibodies against syntaxin-1 (D) & synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Double labeling for syntaxin-1 (G) & PSD-95 (H) shows partial colocalization postsynaptic (I). Scale bars: 10 μm. (J–L) Western blot analysis of synaptosomes (Syn), synaptic cytosolic fraction (Cy), active zone (AZ) & post synaptic density (PSD). Labeled with anti-synaptophysin (J) anti-PSD95 (K) & anti-syntaxin-1 (L). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-556_mouse-monoclonal-psd-95-antibody-6g6-1c9-310202415334924.jpg)
![Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Confocal images of dissociated hippocampal cultures.Double labeling with anti-TUJ1 (A) & anti-VAMP2 (B) demonstrates that VAMP2 is located along the dendrites & gives characteristic punctate labeling (C). Double labeling with antibodies against synaptophysin (P38) (D) & VAMP2 (E) indicates that VAMP2 is colocalized with the presynaptic marker (F). Double labeling for PSD-95 (G) & VAMP2 (H) shows partial colocalization postsynaptic (I). Small, high-resolution pictures of single synapses from (F) & (I) are shown in the right part of these images, respectively. Scale bar: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26488171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-556_mouse-monoclonal-psd-95-antibody-6g6-1c9-310202415395948.jpg)
![Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures.(A) Beta-tubulin (green, anti-TuJ1) labeling of dendrite, after anti-GluA1 external epitope [66] labeling & subsequent plasma membrane permeabilization. Note punctate GluA1 [66] labeling along the dendrite. (B) Synaptophysin (P38, green) & GluA1 [66]. (C) Synaptophysin (green) & GluA2 [66]. (D) Labeling with anti-GluA1. (E) Labeling with anti-GluA1, but the antibody was preincubated with the peptide antigen before staining. (F) Labeling with anti-GluA2. (G) Labeling with anti-GluA2, but the antibody was preincubated with the peptide antigen before staining. (H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) & (I) showing postsynaptic labeling of GluA1. Scale bar (in A, valid for all images): 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26488171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-556_mouse-monoclonal-psd-95-antibody-6g6-1c9-310202416205115.jpg)