STEP Antibody (23E5)


Western Blot: STEP Antibody (23E5) [NB300-202] - Analysis of STEP protein in striatal rat protein homogenates

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

STEP Antibody (23E5) Summary

A synthetic peptide of rat STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR)
NB300-202 is specific for STEP61, STEP46, STEP38, and STEP20.
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.09% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
This STEP (23E5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. By Western Blot, this STEP antibody recognizes bands at ~64-66, 46, 37, and 33 kDa, in rat brain tissues. Neuronal cells have been used for ICC/IF and frozen brain sections have been used for IHC.
Reviewed Applications
Read 1 Review rated 5
NB300-202 in the following application:

Read Publications using
NB300-202 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • 2 publications
  • IP
    7 publications
  • WB
    16 publications

Reactivity Notes

Human, mouse, rat.

Alternate Names for STEP Antibody (23E5)

  • EC
  • FLJ14427
  • Neural-specific protein-tyrosine phosphatase
  • protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
  • protein-tyrosine phosphatase striatum-enriched
  • Striatum-enriched protein-tyrosine phosphatase
  • tyrosine-protein phosphatase non-receptor type 5


STEP (STriatal-Enriched protein tyrosine Phosphatase; PTPN5) is a brain-specific phosphatase enzyme which is regarded as an "LTD protein" because it mediates AMPA receptor internalization during metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD). STEP regulates MAPKs, Src kinases and NMDA receptors which are involved in synaptic plasticity as well as in neuronal cell survival mechanisms. STEP is highly expressed in striatum and is also found in hippocampus, cortex, amygdala, optic nerve, and spinal cord, but not expressed in cerebellum. PKA mediated phosphorylation at Ser-245 site deactivates STEP whereas MAPKs regulated phosphorylation at Thr-255/Ser-268 stabilizes STEP through preventing its proteasomal degradation. Out of four alternatively spliced variants (STEP61, STEP46, STEP38, STEP20), two major isoforms namely STEP61 and STEP46, contains a signature consensus tyrosine phosphatase sequence, [I/V]HCxAGxxR[S/T]G, necessary for its catalytic activity, and a kinase-interacting motif (KIM) essential for substrate binding. During extrasynaptic NMDAR stimulation, a truncated STEP product, STEP33, is generated by calpain-mediated cleavage between residues Ser224 and Leu225 in the KIM domain leading to disruption of STEP's ability to associate with and dephosphorylate its substrates. Subsequently, proteolytic cleavage of STEP after extrasynaptic NMDAR stimulation results in p38 activation, and initiates cell death signaling cascade. STEP participates in dopaminergic, glutamatergic, and nicotinic signaling, thereby playing a key role in psychostimulant addiction and Alzheimer's disease.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P
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Applications: WB, ELISA, ICC/IF
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Publications for STEP Antibody (NB300-202)(29)

We have publications tested in 3 confirmed species: Mouse, Rat, Rat .

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IP, WB.

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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB300-202 Applications Species
Won S, Incontro S, Nicoll RA, Roche KW. PSD-95 stabilizes NMDA receptors by inducing the degradation of STEP61 Proc. Natl. Acad. Sci. U.S.A. 2016 Jul 25 [PMID:27457929] (WB) WB
Rajagopal S, Deb I, Poddar R, Paul S. Aging is associated with dimerization and inactivation of the brain-enriched tyrosine phosphatase STEP. Neurobiology of Aging. 2016 Feb 12 [PMID:27103516] (IP, Rat) IP Rat
Poddar R, Rajagopal S, Shuttleworth CW, Paul S. Zn2+-dependent activation of the Trk signaling pathway induces phosphorylation of the brain-enriched tyrosine phosphatase STEP: molecular basis for Zn2+-induced ERK MAPK activation. J. Biol. Chem. 2015 Nov 16 [PMID:26574547] (WB, IP, Rat) WB, IP Rat
Kim HN, Jang JY, Choi BT. A single fraction from Uncaria sinensis exerts neuroprotective effects against glutamate-induced neurotoxicity in primary cultured cortical neurons Anat Cell Biol 2015 Jun 01 [PMID:26140220] (WB, Rat) WB Rat
Wang Y, Briz V, Chishti A et al. Distinct Roles for u-Calpain and m-Calpain in Synaptic NMDAR-Mediated Neuroprotection and Extrasynaptic NMDAR-Mediated Neurodegeneration. J Neurosci. 2013 Nov 27 [PMID:24285894] (WB, Rat) WB Rat
Deb I, Manhas N, Poddar R et al. Neuroprotective Role of a Brain-Enriched Tyrosine Phosphatase, STEP, in Focal Cerebral Ischemia. J Neurosci. 2013 Nov 6 [PMID:24198371] (IP, WB, Rat) IP, WB Rat
Kim HN, Kim YR, Jang JY et al. Neuroprotective effects of Polygonum multiflorum extract against glutamate-induced oxidative toxicity in HT22 hippocampal cells. J Ethnopharmacol. 2013 Aug 22 [PMID:23973786] (WB, Mouse) WB Mouse
Sun WL, Zelek-Molik A, McGinty JFet al. Short and long access to cocaine self-administration activates tyrosine phosphatase STEP and attenuates GluN expression but differentially regulates GluA expression in the prefrontal cortex. Psychopharmacology (Berl) 2013 Apr 27 [PMID:23624776] (IP, Rat) IP Rat
Shinohara Y, Hosoya A, Yahagi K, Ferecsko AS, Yaguchi K, Sik A, Itakura M, Takahashi M, Hirase H. Hippocampal CA3 and CA2 have distinct bilateral innervation patterns to CA1 in rodents. Eur J Neurosci;35(5):702-710. doi: 10.1111/j.1460-9568.2012.07993.x. 2012 Mar. [PMID:22339771]
Farooq M, Kaswala RH, Kleiman NJ, Kasinathan C, Frederikse PH. GluA2 AMPA glutamate receptor subunit exhibits codon 607 Q/R RNA editing in the lens. Biochem Biophys Res Commun;418(2):273-7. 2012 Feb 10. [PMID:22266371]
Show All 29 Publications.

Reviews for STEP Antibody (NB300-202) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB300-202:
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Images Ratings Applications Species Date Details
reviewed by:
WB Mouse 12/23/2014


ApplicationWestern Blot
Sample TestedSee PMID 22266371


Blocking DetailsSee PMID 22266371


Detection NotesSee PMID 22266371


CommentsIndependent review from antibodyresource.comPublished in PMID 22266371.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STEP Antibody (NB300-202). (Showing 1 - 1 of 1 FAQs).

  1. Is it possible this antibody can detect phospho form of that? Some paper shows that phosphorylated STEP was detected by an pware shift in the mobility of the STEP band. The paper is 17814, The Journal of Neuroscience, November 6, 2013, 33(45):17814 -17826.
    • This antibody may detect both phosphorylated and non-phosphorylated STEP although it has never specifically been tested for this.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol PTPN5

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